不同应激因子对小鼠肝脏金属硫蛋白诱导合成的影响

目的筛选出小鼠肝脏金属硫蛋白（MT）合成量最大的诱导方式。方法从时间-效应和剂量-效应两方面研究了重金属元素（Cd）、微量元素（Zn）、重金属与微量元素的组合（Cd＋Zn）、生理因子（饥饿）及创伤因子等五大类组合应激因子、19种诱导方式对小鼠肝脏中MT诱导合成的影响及效果。结果生理因子诱导MT量最小,饥饿诱导小鼠肝脏MT的量随饥饿程度的加重而增加,但各组间差异不显著（P〉0.05）;创伤因子诱导产生MT的量最高,其诱导量随创伤恢复时间的增加而降低,各组之间差异显著（P〈0.01）,本实验诱导峰值（9.0241±0.6441μmol/g）出现在创伤后6 h;重金属元素和微量元素诱导量居中,且两者混合诱导量比单独诱导量之和要大。结论成功筛选出诱导小鼠肝脏MT合成最有效的因子和最佳时间,为进一步大量合成MT及研究其功能等奠定基础。

Effects of Different Stress Factors on Induction of Metallothionein Synthesis in Mouse Liver

Objective To developan optimal treating way to induce the most production of metallothionein in mouse liver.Methods Trauma,starvation,zinc（Zn）,cadmium（Cd）,Zn-Cd mixture,all together five kinds of stress factors and 19 treating ways,were used as inducers to enhance the production of metallothionein in Kunming mouse liver,designed according to the duration-effect and dose-effect relationship.Results The production of metallothionein induced by starvation was the least among all inducers,the longer the mouse-hungry time the higher production of metallothionein was induced,but there were no significant differences（P〉0.05） between each two groups.Among all stress factors,the production of metallothionein induced by trauma was the highest,the longer trauma-recovery time the lower production of metallothionein was induced.The production peak（9.0241±0.6440 μmol/g） of metallothionein induced came out 6 hours after trauma,and there were significant differences（P〈0.01） between each two groups.The production of metallothionein induced by Zn and Cd was less than that of Zn-Cd mixture,but all of them lied between those of trauma and starvation groups.Conclusion The most effective stress factor and the best duration of metallothionein synthesis induced in mouse liver have been successfully selected,which will contribute to mass synthesizing metallothionein and researches on its function.