常规实验操作对Wistar大鼠的影响

目的常规实验操作对Wistar大鼠的影响。方法采用Wistar大鼠,分别进行腹腔注射、肌内注射、灌胃、尾静脉切割采血、剪尾采血和固定操作,连续处理7d后,测定各组大鼠神经内分泌、免疫、血液系统主要指标及肝脏hsp72mRNA表达量,并与对照组比较,分析这些操作的影响。结果与对照组相比,腹腔注射组、肌内注射组和灌胃组的皮质酮(CORT)、γ-干扰素(IFN-γ)、γ-干扰素/白介素-4(IFN-γ/IL-4)比值极显著升高,白细胞总数(WBC)、中性粒细胞比例(WLCR)、C-反应蛋白(CRP)、β-内啡肽(β-EP)及白介素-2(IL-2)有不同程度的显著降低。与对照组相比,尾静脉切割采血组的CORT、IFN-γ、IFN-γ/IL-4比值极显著升高,WBC、β-EP有不同程度的显著降低;剪尾采血组的CORT、IFN-γ、IFN-γ/IL-4比值有不同程度的显著升高,β-EP、IL-2、CRP极显著降低;固定组的CORT、IFN-γ/IL-4比值、IL-2、CRP极显著升高,WBC、β-EP、IL-4有不同程度的显著降低。与对照组相比,所有实验操作均使热休克蛋白(HSP)hsp72mRNA表达极显著升高。此外,与对照组相比,肌内注射组的红细胞数(RBC)和血红蛋白含量(HGB)显著升高,尾静脉切割和剪尾采血组的HGB和红细胞压积(HCT)极显著降低,固定组的RBC、HGB和HCT均极显著升高。结论本研究中涉及的常规实验操作均引起了大鼠极显著的应激反应,并造成了大鼠神经内分泌系统的紊乱及免疫功能的抑制,从而对动物造成了恶性应激。同时这些操作可能会对涉及的神经内分泌及血液学指标的相关研究产生背景性干扰。

Negative Impact of Routine Experimental Procedures on Wistar Rats

Objective To study the influence of routine experimental procedures on Wistar rats. Methods Intraperitoneal injection （ ip）,intramuscular injections （ im）,intragastric injection （ ig）,blood sampling by tail vein incision （ tv）,blood sampling by tail tip cutting （ tc） and restraint procedures were selected in this experiment. Each procedure was respectively done on Wistar rats. After continuously handled for seven days,the main neuroendocrine, immune and haematological indexes were measured to study the effects of these procedures on the rats. Results Compared with the control group,CORT,IFN-γ,IFN-γ /IL-4 were significantly increased and WBC,WLCR,CRP,β-EP,IL-2 were significantly decreased in the ip,im and ig groups. Compared with the control group,CORT,IFN-γ,IFN-γ /IL-4 were significantly increased and WBC,β-EP were significantly decreased in the tv group; CORT,IFN-γ,IFN-γ /IL-4 were significantly increased and β-EP,IL-2,CRP significantly decreased in the tc group; CORT,IFN-γ /IL-4,IL-2,CRP significantly increased, and WBC, β-EP, IL-4 significantly decreased in the restraint group. Hsp72 mRNA was significantly increased in all the experimental groups. Furthermore,compared with the control group,RBC and HGB were significantly increased in the im group,HGB and HCT significantly decreased in the tv and tc groups,RBC,and HGT and HCT significantly increased in the restraint group. Conclusion Routine experimental procedures cause severely stress reactions,and lead to neuroendocrine dysfunction and immunosuppression in rats. It is of importance that those stress conditions may lead to considerable background-interference on some experimental neuroendocrine,immunological and haematological researches.