西藏小型猪线粒体D-loop区及微卫星多态性的遗传学分析

目的通过分析西藏小型猪线粒体控制区(D-loop区)及微卫星位点的遗传多态性,检测西藏小型猪的遗传背景,从而为其作为实验动物提供分子生物学方面的可靠依据。方法利用特异性引物对西藏小型猪的线粒体D-loop区及10个具有多态性的微卫星位点进行扩增,割胶纯化并对线粒体D-loop区进行测序,另外采用聚丙烯酰胺凝胶电泳的方法分离微卫星位点的等位基因。结果西藏小型猪线粒体D-loop区全序列没有多态性,微卫星位点则具有高度的遗传多态性和杂合度,分别为0.584和0.573。结论西藏小型猪线粒体基因组无多态性,证明其在母系进化和遗传上与其他猪种较为一致,本实验所用的西藏小型猪生长于一个封闭的环境,导致其微卫星位点遗传多态性的中低度水平。

Genetic Analysis of the Polymorphism of mtDNA D-loop and Microsatellite Loci in Tibet Mini-Pigs

Objective To study the genetic polymorphism of mtDNA D-loop and microsatellite loci in Tibet mini-pigs in order to evaluate their background and lay a molecular basis.Methods Differential primers to amplify the mtDNA D-loop and ten microsatellite loci in the Tibet mini-pigs were used.Then mtDNA Dloop from gel was purified and sequenced.PAGE was used to separate the alleles of microsatellite loci in the Tibet mini-pigs.Results Analysis of polymorphism of mtDNA D-loop in Tibet mini-pigs showed that there was no change in the mtDNA D-loop.The polymorphism of 6 microsatellite loci in 30 Tibet mini-pigs was high with a mean polymorphism information content(PIC) of 0.584 and mean rate of heterozygosity(H) of 0.573.Conclusion MtDNA D-loop in Tibet mini-pigs presents no diversity which proves that it is consistent with other seeds of pigs in evolution and inheritance.The average rate of microsatellite loci in Tibet mini-pigs proves that this seed is pure.