大鼠运动性疲劳模型的建立

目的建立大鼠运动疲劳模型,观察运动疲劳对大鼠各项生理、生化指标的影响。方法20只大鼠随机分为正常对照组和运动疲劳模型组,运动疲劳模型组大鼠每日按照方案进行锻炼。实验结束后大鼠检测相关指标：血清MDA含量和红细胞SOD活性,肝脏与骨骼肌MDA含量、SOD活性,骨骼肌线粒体游离钙离子含量,骨骼肌线粒体膜电位,下丘脑神经递质。电镜观察骨骼肌线粒体细微结构。结果运动疲劳模型组大鼠造模2周以后其血清、肝和骨骼肌MDA含量均有显著升高,红细胞和骨骼肌SOD活性均有显著降低,骨骼肌线粒体膜电位显著性降低,骨骼肌线粒体游离Ca2＋含量有显著性降低,下丘脑GABA、5-HT含量有显著升高,下丘脑DA、ACh含量有显著性下降,电镜观察显示骨骼肌超微结构改变并以线粒体改变较为明显。结论大鼠跑台运动2周可造成运动疲劳模型,并造成大鼠骨骼肌线粒体损伤。

Establishment of a Rat Model of Exercise-Induced Fatigue

Objective To study the influence of exercise-induced fatigue on rat physical and biochemical indexes.Method Twenty rats were randomly divided into two groups： the control and model groups.The model group was given treadmill exercise according to the scheme.Related indexes were detected after training.The serum content of MDA,SOD activity of erythrocytes,content of MDA in liver and skeletal muscle,SOD activity of liver and skeletal muscle,free calmodulin in mitochondria of skeletal muscle cells,mitochondrial membrane potential,and hypothalamic neurotransmitters were measured.Myocardial specimens were taken for electron microscopic examination.Result Compared with the control group,the exercise-induced fatigue rats＇ contents of MDA in serum,liver and skeletal muscles increased significantly,the SOD activity of skeletal muscle and erythrocytes markedly decreased,mitochondrial membrane potential markedly decreased,content of GABA and 5-HT in hypothalamus increased significantly,and the contents of DA and ACh in hypothalamus decreased significantly.The electron microscopic observation revealed that the skeletal muscle utrastructure was changed,among others,the changes of mitochondria were most noticeble.Conclusion A rat model of exercise-induced fatigue can be well established by two weeks treadmill training.This rat model shows apparent mitochondrial damages supporting the skeletal muscle injury induced by fatigue.