家蚕近交系遗传纯度的RAPD检测

目的分析家蚕近交系IS-c108A的遗传纯度,为家蚕实验动物化的培育工作提供指导。方法应用经过筛选的20条随机引物对家蚕近交系IS-c108A(F10)的3个蛾区各30个个体和该近交系的亲本系统c108、对照实用化品种871各30个个体的基因组DNA进行RAPD扩增,计算个体间和蛾区间的相似系数及遗传距离。结果家蚕近交系IS-c108A(F10)的3个蛾区内的多态性带频率分别为1.807%、1.841%、1.841%,平均为1.830%;起点亲本c108个体间多态性带频率为7.207%,对照品种871个体间的多态性带频率为7.08%;而近交系IS-c108A与c108之间的多态性带频率为49.20%,c108和871品种之间的多态性带频率为58.33%。家蚕近交系IS-c108A10的3个蛾区内个体之间遗传相似系数的平均值分别为0.99581、0.99555、0.99551,总平均为0.99562。结论家蚕近交系IS-c108A(F10)已具有较高的遗传纯合度,家蚕具有易于获得高纯的有利条件。

Determination of Genetic Purity of Bombyx mori Inbred Strain by RAPD

Objective To analyze the genetic purity of silkworm (Bombyx mori) inbred strain IS-c108A for directing the breeding of silkworm as a laboratory animal. Methods 20 selected single primers were used to amplify randomly genomic DNA of 30 individuals in each batch of the inbred strain IS-c108A, parent strain c108 of this inbred strain and the control strain 871. The amplified fragment bands were analyzed to calculate the similarity index and the genetic distance among the individuals and batches. Results The frequency of polymorphic bands in the three batches of the inbred strain IS-c108A were 1.807%, 1.841% and 1.841%, respectively, and the average was 1.830%. The frequency of polymorphic bands was 7.207% between 30 individuals of the parent strain c108 of this inbred strain,while it was 7.08% between individuals of the control strain 871. The frequency of polymorphic bands between the inbred strain IS-c108A and c108 was 49.20%,while it was 58.33% between c108 and 871. Based on RAPD patterns, the calculated similarity index between 30 individuals in each batch of the inbred strain IS-c108A were 0.99581, 0.99555 and 0.99551, respectively, the average was 0.99562. Conclusion All the results implied that the inbred strain IS-c108A of silkworm has reached a high degree of genetic purity, and the silkworm has the advantage to obtain an inbred strain with high degree of purity easily.