山医群体近交系中国地鼠的G-显带核型和自发畸变率的研究

目的　阐明山医群体近交系中国地鼠G 显带核型和自发畸变率 ,完善中国地鼠的背景资料。方法　采用地鼠骨髓制备和G 显带法。结果　从 14只地鼠的 30个G 显带细胞中 ,7个G 显带细胞被选作模型分析。根据特有带型来识别各号染色体 ,绘制了模式图。此外 ,用常规Giemsa染色分析了 80 0个中期细胞 ,发现染色体断裂为 0 2 5 % ,无着丝粒畸变和不平衡易位均为 0 0 5 % ,自发畸变率很低。结论　山医群体近交系中国地鼠G 显带的识别为结构异常和基因作图提供了科学依据。

Study on G-banding Pattern and Spontaneous Aberration Frequency in an Inbred Line of Chinese Hamster of Shanyi Colony

ObjectiveTo clarify the G -banding pattern and spontaneous aberration frequencies of an inbred line of Chinese hamster of shanyi colony. Methods The bone marrow chromosome preparation and G -banding pattern for Chinese hamster were applied. Results Of the 30 G -banding cells from 14 Chinese hamsters 7 cells were selected for banding analysis. The chromosomes were distinguished by characteristic banding patterns. In addition,800 metaphase cells were analysed by conventional Giemsa stain. The results showed that break was 0.25%, acentric aberration and translocation were 0.05%. The spontaneous aberration frequencies were very low. Conclusion The discernment of G -banding pattern of an inbred line of Chinese hamster is for providing scientific basis to study on abnormal structure and gene map.