| 应用RAPD方法对近交系小鼠进行遗传检测的研究 |
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| 引用本文： | 王洪,岳秉飞,刘双环,左琴,贺争鸣,邢瑞昌.应用RAPD方法对近交系小鼠进行遗传检测的研究[J].中国实验动物学报,2003,11(3):184-186. |
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| 作者姓名： | 王洪 岳秉飞 刘双环 左琴 贺争鸣 邢瑞昌 |
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| 作者单位： | 中国药品生物制品检定所,北京,100050 |
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| 摘 要： | 目的 为实验室日常检测近交系小鼠的遗传背景提供一种分子生物学方法。方法 用 6条随机引物对 6个品系近交系小鼠基因组DNA进行PCR扩增。结果 6条随机引物中p2、p3、p5和p6四引物扩增的条带差异较为明显。结论 RAPD方法是一种有效的近交系小鼠遗传检测手段
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| 关 键 词： | 小鼠 近交系 聚合酶链反应 遗传学技术 |
| 文章编号： | 1005-4847(2003)03-0184-03 |
| 修稿时间： | 2002年11月25 |
Studying RAPD Method for Genetic Monitoring in Inbred Strain Mice |
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| WANG Hong,YUE Bing-fei,LIU Shuang-huan,ZUO Qin,HE Zheng-ming,XING Rui-chang.Studying RAPD Method for Genetic Monitoring in Inbred Strain Mice[J].Acta Laboratorium Animalis Scientia Sinica,2003,11(3):184-186. |
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| Authors: | WANG Hong YUE Bing-fei LIU Shuang-huan ZUO Qin HE Zheng-ming XING Rui-chang |
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| Abstract: | Objective To study a new method for inbred strain mice genetic monitoring.Method 6 random primers were used to amplify DNA in 6 inbred strains of mice (TA1, TA2, T739, C57BL/6,615 and Scid). Results The amplified band patterns were obviously different in the products of 4 primers(p2,p3,p5 and p6).Conclusion The results indicated that different strains of mice could be easily distinguished by RAPD method. |
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| Keywords: | Mice Inbred strains Polymerase chain reaction Genetic techniques |
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