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| 镉染毒大鼠胫骨骨微结构的活体Micro-CT观察 | |
| 引用本文： | 秦波音,陈晓,朱国英,周文江.镉染毒大鼠胫骨骨微结构的活体Micro-CT观察[J].中国实验动物学报,2011,19(6):517-521. |
| 作者姓名： | 秦波音 陈晓 朱国英 周文江 |
| 作者单位： | 1. 上海市公共卫生临床中心,上海,201508 2. 复旦大学放射医学研究所,上海,200032 3. 上海市公共卫生临床中心,上海201508;复旦大学药学院动物中心,上海201203 |
| 摘 要： | 目的应用Micro-CT研究不同剂量CdCl2染毒后大鼠胫骨松质骨骨小梁密度和骨微结构的差异。方法 24只3月龄雄性SD大鼠随机分成4组,对照组、低剂量组、中剂量组和高剂量组,分别背部皮下注射生理盐水(0.5 mL)和0.1、0.5、1.5 mg/(kg.bw)CdCl2,每周称体重,并根据体重调整注射量。染毒后第8周对所有大鼠左侧胫骨近端进行活体Micro-CT扫描及三维重建。选取距生长板远端0.8、1.2 mm厚的骨组织为感兴趣区域,进行骨形态计量分析。结果镉染毒组大鼠体重和对照组相比有不同程度下降,高剂量组和其他组相比差异有显著性(P<0.05);镉染毒组大鼠骨密度、骨体积分数及骨小梁数量较对照组明显减少,高剂量组与对照组相比差异有显著性(P<0.01);镉染毒组大鼠胫骨骨小梁分离度及骨结构模型指数都较对照组有明显增高,并且随着染毒剂量的增加而升高。二维及三维图象显示,镉作用后大鼠胫骨骨髓腔增宽,骨量、骨小梁数量及骨小梁连接明显减少,板状骨数量减少,杆状骨数量增加。结论镉染毒对大鼠胫骨骨量及骨微观结构有明显损害。 |
| 关 键 词： | 镉 大鼠 骨微结构 Micro-CT |
Micro-CT analysis of the tibia microstructure in live male rats exposed to cadmium |
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| QIN Bo-yin,CHEN Xiao,ZHU Guo-ying,ZHOU Wen-jiang.Micro-CT analysis of the tibia microstructure in live male rats exposed to cadmium[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(6):517-521. | |
| Authors: | QIN Bo-yin CHEN Xiao ZHU Guo-ying ZHOU Wen-jiang |
| Institution: | QIN Bo-yin1,CHEN Xiao2,ZHU Guo-ying2,ZHOU Wen-jiang1,3(1.Shanghai Public Health Clinical Center Affiliated to Fudan University,Shanghai 201508,China,2.Institute of radiation Medicine,Fudan University,Shanghai 200032,3.Animal Center,School of Pharmacy,Shanghai 201203) |
| Abstract: | Objective To investigate the effects of cadmium on bone microstructure in live male rats by micro-CT imaging.Methods Twenty-four 3-month-old Sprague-Dawley male rats were randomly divided into 4 groups which were given CdCl2 by subcutaneous injection at a doses of 0(sodium chloride),0.1,0.5 and 1.5 mg/kg body weight,respectively.At the 8th week,micro-CT analysis was performed on the proximal tibia for bone microstructure evaluation.Trabecular bone was reconstructed and selected for analysis within a conform... |
| Keywords: | Cadmium Rat Bone microstructure Micro-CT |
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