大分子葡萄糖酐所致血瘀模型小鼠中偶氮蓝渗出量与血瘀程度的相关性

目的探讨大分子葡萄糖酐血瘀模型小鼠偶氮蓝渗出量和血瘀程度的相关性。方法用0.5%偶氮蓝将大分子葡萄糖酐配制成10%、5%、2.5%的浓度,尾静脉注射,0.1mL/10g,分别在注入后0.5、1、2h对造模小鼠耳、舌的颜色进行观察,并用丙酮无水硫酸钠溶液将耳组织中的偶氮蓝提出,在590μm测定各实验组小鼠耳廓偶氮蓝含量。结果实验结果表明,不同浓度的大分子葡萄糖酐所致血瘀模型的血瘀程度明显不同,同单用偶氮蓝组小鼠相比,大分子葡萄糖酐10%、5%、2.5%的浓度造模小鼠的耳、舌的颜色明显呈现蓝色,随着剂量的增加偶氮蓝渗出量明显增加(P<0.05或0.01),在尾静脉注射后0.5h,造模小鼠耳、舌的颜色呈现点状或片状蓝色,在尾静脉注射后1h,造模小鼠耳、舌的颜色明显呈现蓝色,在尾静脉注射后2h,造模小鼠耳、舌的颜色明显消退。结论表明大分子葡萄糖酐所致血瘀模型的血瘀程度和偶氮蓝渗出量明显相关,呈现明显的量效和时效关系。

Quantitative Analysis of the Exudation of Evans Blue in Mouse Model of Blood Stasis Induced by Macromolecular Dextran

Objective To explore the correlation between the exudation amount of Evans blue in mouse model of blood stasis induced by macromolecular dextran and the degree of blood stasis.Method Preparing 10%,5% and 2.5% dextran solution with 0.5% Evens blue in normal saline.Mice were injected the preparation into tail vein,0.1 mL/10 g.The skin colour of ear and tongue was examined.Evens blue was extracted from the ears by anhydrous acetone and Na2SO4 solution and determined at 590 nm by ultraviolet spectrophotometer at 0.5,1 and 2 hours after dextran injection.Results Compared with the Evens blue-group mice,the whole skin of tongue,in 10%,5% and 2.5% dextran groups appeared blue and the blue color became deeper along with the increasing content of dextran(P<0.05 or P<0.01).At 0.5 hour after dextran injection,the skin of tongue,mice appeared plaque blue.One hour later,the whole skin appeared obvious blue,while its blue faded away about two hours later.Conclusion The degree of blood stasis induced by macromolecular dextran is apparently correlated to the exudation amount of Evens blue,and the degree of blood stasis is regularly related with the content and time of exudation amount of Evens blue.