| 恒河猴感染SARS-CoV的病毒学、血清学检测 |
| |
| 引用本文： | 魏强,王健伟,张扬清,鲍琳琳,涂新明,蒋虹,高红,丛喆,朱华,邓巍,佟巍,张兵林,黄澜,刘亚莉,秦川.恒河猴感染SARS-CoV的病毒学、血清学检测[J].中国实验动物学报,2005,13(3):144-146. |
| |
| 作者姓名： | 魏强 王健伟 张扬清 鲍琳琳 涂新明 蒋虹 高红 丛喆 朱华 邓巍 佟巍 张兵林 黄澜 刘亚莉 秦川 |
| |
| 作者单位： | 1. 中国医学科学院,中国协和医科大学,实验动物研究所,北京,100021
2. 中国疾病预防控制中心病毒病研究所,北京,100052 |
| |
| 基金项目： | 863课题(编号:2003AA208205，2003AA208213)。 |
| |
| 摘 要： | 目的对感染SARS-CoV的8只恒河猴进行病毒学、血清学指标检测。方法SARS-CoV经鼻腔接种8只恒河猴,在感染的第1天开始到5、7、10、15、20、30和60天分别安乐处死时,不同时间取咽拭子、血液和脏器,进行病毒分离,RT-PCR检测和抗体测定。结果RT-PCR证实感染病毒检出时间为5~16d,8只猴中的5只分离到了病毒,感染15d后可检测到抗体。结论感染SARS-CoV的恒河猴不仅出现与SARS患者类似的临床和病理学改变,也在一定时期内排毒,出现特异免疫反应,这些指标均可作为药物筛选、疫苗评价等方面的重要参数。
|
| 关 键 词： | SARS-CoV 恒河猴 血清学 病毒学 |
| 文章编号： | 1005-4847(2005)03-0144-03 |
| 修稿时间： | 2004年4月1日 |
Virologic and Serologic Detection of SARS-CoV in Infected Rhesus Monkeys |
| |
| WEI Qiang,WANG Jian-wei,ZHANG Yang-qing,BAO Lin-lin,TU Xin-ming,JIANG Hong,GAO Hong,CONG Zhe,ZHU Hua,DENG Wei,TONG Wei,ZHANG bing-lin,HUANG Lan,LIU Ya-li,QIN Chuan.Virologic and Serologic Detection of SARS-CoV in Infected Rhesus Monkeys[J].Acta Laboratorium Animalis Scientia Sinica,2005,13(3):144-146. |
| |
| Authors: | WEI Qiang WANG Jian-wei ZHANG Yang-qing BAO Lin-lin TU Xin-ming JIANG Hong GAO Hong CONG Zhe ZHU Hua DENG Wei TONG Wei ZHANG bing-lin HUANG Lan LIU Ya-li QIN Chuan |
| |
| Institution: | WEI Qiang1,WANG Jian-wei2,ZHANG Yang-qing1,BAO Lin-lin1,TU Xin-ming1,JIANG Hong1,GAO Hong1,CONG Zhe1,ZHU Hua1,DENG Wei1,TONG Wei1,ZHANG bing-lin1,HUANG Lan1,LIU Ya-li1,QIN Chuan1 |
| |
| Abstract: | Objective To study virological and serological detection of SARS-CoV in the infected Rhesus monkeys. Methods Eight rhesus monkeys were infected with SARS-CoV inoculated into the nasal cavity, and euthanatized at 5, 7, 10, 15,20, 30 and 60 days after inoculation, respectively. Throat swabs and blood samples were collected periodically for detecting the SARS-CoV and antibodies by RT-PCR, virus isolation and ELISA. Results Infections in the monkeys have been successfully established by SARS-CoV inoculation. The SARS virus RNA was detected on 5-16 days after inoculation using nested RT-PCR in throat swab samples. Viruses were isolated in 5 out of 8 infected monkeys. The SARS coronavirus-specific IgG was positive from 15 th day post infection. Conclusion The duration of PCR detection, virus isolation and antibody production may be used as important criteria for anti-SARS drug studies and vaccine evaluation. |
| |
| Keywords: | SARS-CoV Rhesus monkeys serology Virology |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
