| SARS冠状病毒感染Lewis大鼠模型的建立 |
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| 引用本文： | 朱华,高虹,邓巍,张扬清,鲍琳琳,黄澜,刘亚莉,秦川.SARS冠状病毒感染Lewis大鼠模型的建立[J].中国实验动物学报,2008,16(4):261-264. |
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| 作者姓名： | 朱华 高虹 邓巍 张扬清 鲍琳琳 黄澜 刘亚莉 秦川 |
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| 作者单位： | 中国医学科学院中国协和医科大学实验动物研究所,北京100021 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的通过观察SARS-CoV感染Lewis大鼠后,病理学、免疫学以及病毒的复制与外排情况变化,探讨其能否作为有效的SARS动物模型。方法SARS病毒感染9只Lewis大鼠,在感染后不同时间安乐死动物,应用光镜对动物的各脏器进行病理观察研究;用病毒分离和RT-PCR方法检测病毒外排与复制的情况;用ELISA法检测动物产生特异性抗体情况。结果在SARS-CoV感染Lewis大鼠后,肺组织出现一定的与人类SARS疾病相似的病理改变,在动物体内可检测到活病毒或病毒核酸,并可检测到特异性IgG抗体的存在。结论Lewis大鼠出现了特异的免疫反应及特征性病理改变,可做为灵长类SARS动物模型的有益补充用于SARS发病机理及疫苗的研发等。
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| 关 键 词： | 严重急性呼吸综合症 Lewis大鼠 动物模型 |
Establishment of a Lewis Rat Model of SARS-CoV Infection |
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| ZHU Hua,GAO Hong,DENG Wei,ZHANG Yang-qing,BAO Lin-lin,HUANG Lan,LIU Ya-li,QIN Chuan.Establishment of a Lewis Rat Model of SARS-CoV Infection[J].Acta Laboratorium Animalis Scientia Sinica,2008,16(4):261-264. |
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| Authors: | ZHU Hua GAO Hong DENG Wei ZHANG Yang-qing BAO Lin-lin HUANG Lan LIU Ya-li QIN Chuan |
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| Institution: | (Institute of laboratory Animal Science, Chinese Academy of Medical sciences ＆ Peking Union Medical College, Bejing 100021, China) |
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| Abstract: | Objective To establish a sensitive Lewis rat model of SARS-CoV infection based on immunology, serology and virus replication test. Method Nine Lewis rats were infected with SARS-CoV through the nasal cavity, sacrificed at certain time points after inoculation, and blood serum and tissue samples were collected for histopathological examination, test for immune response and SARS-CoV rephcation in vivo. Result Similar pathological changes were observed in the SARS-CoV infected Lewis rats. Virus replication in the rats was identified by virus isolation and RT-PCR. Immune response in vivo was identified by IgG measurement. Conclusion Lewis rat can be used to supplement the primate SARS animal model in studies on SARS-CoV replication and vaccine development. |
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| Keywords: | Severe acute respiratory syndrome Lewis rat Animal model |
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