二维斑点追踪技术评价犬心梗后自体骨髓CD34＋干细胞移植对心肌功能的影响

目的应用二维斑点追踪成像超声心动图（2D-STE）,评价犬心梗后自体骨髓CD34＋干细胞移植对心肌功能的影响。方法 12只杂种犬行冠脉左前降支结扎术,导致前壁心肌梗死,随机分为两组,A组为对照组,结扎术后两周二次开胸手术,经心肌注射磷酸盐缓冲液（PBS）1 mL;B组为治疗组,结扎术后两周二次开胸手术,经心肌注射含自体骨髓CD34＋干细胞的磷酸盐缓冲液1 mL。应用STE对12只犬结扎术前、术后左室短轴基底段及心尖段心室节段径向应变（RS）、圆周方向应变（CS）以及局部心肌旋转（Rot）进行分析,并对对照组和治疗组治疗后的RS、CS及Rot变化进行比较。结果心肌梗死后梗死节段的RS、CS以及Rot均下降,治疗后治疗组梗死段RS及Rot较对照组好转。结论 STE能够评价左室短轴局部心肌的收缩功能,心肌梗死后梗死段短轴各方向应变减低,自体骨髓CD34＋干细胞移植能够提高局部心肌的收缩功能。

Two-Dimensional Speckle Tracking Echocardiographic Evaluation of the Effects of Autologous Bone Marrow CD34 + Stem Cell Transplantation on Cardiac Function in Dogs with Myocardial Infarction

Objective To evaluate the cardiac function after autologous bone marrow CD34＋ stem cell transplan-tation by two-dimensional speckle tracking echocardiography（2D-STE） in dogs with myocardial infarction.Methods The left anterior descending coronary artery was ligated to produce myocardial infarction model.Twelve dogs were randomly di-vided into two groups.Group A was the control group,receiving 1 mL myocardial injection of phosphate buffer solution（PBS）.Group B was the treatment group treated with myocardial injection of autologous bone marrow CD34 ＋ stem cells in 1 mL phosphate buffer.Using of 2D-STE,radial strain（RS） circumferential strain（CS） and regional myocardial rotation（Rot） of apical and basal segments in the left ventricular anterior wall were analyzed and compared,in short axis of 12 dogs before and after operation.Results RS,CS and Rot of infracted segments were decreased after myocardial infarction.Comparing with group A,RS and Rot of transplantation segments in the group B were significantly increased.Conclusion 2D-STE can be used to evaluate the left ventricular fractional regional myocardial contractile function.The short axis in all directions of infarct segment in myocardial infarction was reduced.Autologous bone marrow CD34 ＋ stem cell transplan-tation can improve the regional myocardial contractile function.