不同来源H22细胞株的生物学特性比较研究

目的　对不同研究单位保存的小鼠肝癌H2 2 细胞株的基本生物学特性进行比较研究。方法　从国内3个不同研究单位 (武汉大学典型培养物保藏中心 ,山东省医学科学院药物研究所以及中国医学科学院药物研究所 )引进小鼠肝癌H2 2 瘤株 (分别简称武汉H2 2 、山东H2 2 、北京H2 2 ) ,KM小鼠腹腔传代 ,然后对其体外培养特性、体内生长特征、病理形态、治疗学特征进行了比较分析。结果　 3株H2 2 细胞在体内、体外生长速度明显不同 ,武汉H2 2 生长速度较其他瘤株快 (P <0 .0 1) ,接种一致性好 ;而山东H2 2 则在 10 4 个细胞 只小鼠时即可接种成功。荷瘤小鼠体内未发现明显转移灶 ,但肿瘤形态各不相同。环磷酰胺治疗时 ,山东H2 2 抑瘤率最高为 74 6%。结论　不同研究单位H2 2 瘤株的生物学特性有很大不同 ,虽为同一起源 ,但经不同单位保存使用一定时间后 ,它们之间的生物学特性已存在较大的差异

The Comparison in Biological Characteristics among Murine Hepatoma 22 Cell Lines from Different Institutes

Objective\ To compare the biological characteristics of H 22 cell lines from different institutes. Methods\ H 22 cell lines from three institutes,that is,Beijing H 22 from Institute of Pharmacology of Chinese Medical Academy,Shandong H 22 from Institute of Pharmacology of Shandong Medical Academy and Wuhan H 22 from China Center for Type Culture Collection in Wuhan,were kept in KM mouse abdominal cavity,then their comparative analyses of culture behavior in vitro, growth feature in vivo, pathomorphism and therapeutic outcome were carried on. Results\ The growing speeds of three cell lines in vitro and in vivo were different markedly.The growing speed of Wuhan H 22 was fastest ( P<0.01 ) with the best consistency.Shandong H 22 could inoculate successfully at lowest cell concentration of 10\ 4 cells per mouse.The tumor conformations of different H 22 were different.The mice bearing H 22 tumor were treated with CTX, showing the highest neoplastic inhibition rate (74.6%)in mice with Shandong H 22 . Conclusions\ The biological characteristics of H 22 from different institutes were markedly different,though they were of the same origin.