除草剂乐草隆对红鲫的遗传毒性研究

目的　探讨除草剂乐草隆对红鲫的遗传毒性。方法　用单细胞凝胶电泳检测不同浓度的乐草隆对红鲫外周血淋巴细胞DNA的损伤作用。结果　乐草隆致毒红鲫的淋巴细胞DNA的迁移度均较阴性对照组高 (P<0 0 5 ) ,在一定浓度范围内 (0～ 7 0 0mg L)DNA损伤程度与浓度呈正相关 (r=0 982 ,P <0 0 1)。在 12h、2 4h、4 8h、96h、10d实验组DNA损伤程度均有增强的趋势。结论　乐草隆对红鲫具有一定的遗传毒性

Genetic Toxicity in Lymphocyte of Red Crucian Carp Exposed to the Herbicide LeCaoLong

Objective To study the effects of herbicide LeCaoLong on DNA damage of red crucian carp lymphocyte in vivo . Method Single cell gel electrophoresis (SCGE) assay was used to detect DNA single strands breaks (DNA SSBs) when red crucian carps were exposed to different concentration of LeCaoLong. Results At the concentration of 0 88, 1 75, 3 50, 7 00 mg/L, LeCaoLong could induce DNA damage in the peripheral blood lymphocyte of red curcian carp, the comet cell tail length of every group exposed to LeCaoLong was longer than the control group( P<0 05 ). The DNA damage was signficantly positively correlated with concentrations in the range of 0-7 0 mg/L,( r=0 982,P<0 01 ), when red crucian carps exposed to LeCaoLong for 12 h,24 h,48 h,96 h and 10days. The comet cell rates of every concentration group were higher than relative controls. Conclusion All the results suggest that the herbicide can cause significant genetic damage to the lymphocyte of red crucian carp at certain concentration and time.