| 提高制备转基因小鼠效率的研究 |
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| 引用本文： | 程萱,陈红星,杨晓,谭晓红,黄培堂.提高制备转基因小鼠效率的研究[J].中国实验动物学报,2001,9(3):160-163. |
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| 作者姓名： | 程萱 陈红星 杨晓 谭晓红 黄培堂 |
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| 作者单位： | 军事医学科学院生物工程研究所 |
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| 基金项目： | 国家高技术研究发展资助(Z21-03-01和102-08-08-02) |
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| 摘 要： | 目的提高制备转基因动物的效率.方法着重对注射DNA的制备,高质量受精卵的获得,显微注射及胚胎移植各步骤中的主要影响因素进行了优化.结果移植后出生的小鼠经PCR及Sonthern-Blot检测,从最初的146只仔鼠中检测到2只阳性鼠,提高到由34只仔鼠中检测到10只阳性鼠,阳性鼠概率由1.4%基本稳定增加到30%.结论通过技术改良后,转基因效率得到明显提高,为从事转基因动物方面的研究建立了稳定的技术平台.
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| 关 键 词： | 转基因小鼠 微量注射 |
| 文章编号： | 1005-4847(2001)03-0160-04 |
| 修稿时间： | 2000年7月2日 |
Study on improving efficiency of producing transgenic mouse |
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| CHENG Xuan,CHEN Hongxing,YANG Xiao,TAN Xiaohong,HUANG Peitang.Study on improving efficiency of producing transgenic mouse[J].Acta Laboratorium Animalis Scientia Sinica,2001,9(3):160-163. |
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| Authors: | CHENG Xuan CHEN Hongxing YANG Xiao TAN Xiaohong HUANG Peitang |
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| Abstract: | Objective To improve the efficiency of producing transgenic mice.Methods The major steps were the preparation of DNA fragments for microinjection,the preparation of eggs with high quality,and the microinjection and implantation optimized.Results The born mice were examined by PCR and Southern Blot.At the beginning,we got 2 transgenic mice in 146 born mice.After improvement,we got 10 transgenic mice from 34 born mice.The ratio of transgenic founder mice was improved from 1.4% to 30%.Conclusion The technical platform for transgenic research has been establised due to the great improvement of the efficiency of producing transgenic mice. |
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| Keywords: | Transgenic mouse Microinjections |
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