IRM-2近交系小鼠的G-显带核型和自发畸变率的研究

目的阐明新型IRM-2近交系小鼠G-显带核型和自发畸变率.方法采用小鼠骨髓制备和G-显带法.结果从20只小鼠的50个G-显带细胞中,10个G-显带细胞被选作模型分析.根据特有带型来识别各号染色体,描述了带型特征,测量了相对长度和标准差,绘制了模式图.对于1、6与X,4与5,9、13与14等带型较相似的染色体提出了一些识别要点.此外,用常规Giemsa染色分析了1200个中期细胞,发现染色体断裂为0.33%,无着丝粒畸变和不平衡易位均为0.08%,自发畸变率很低.结论新型IRM-2近交系小鼠G-显带的识别为结构异常、辐射效应、肿瘤研究和基因作图提供了科学依据.

Study on G-banding Pattern and Spontaneous Aberration Frequency in an Inbred Line Mouse IRM-2

Objective To clarify the G banding pattern and spontaneous aberration frequencies of a new inbred line mouse IRM 2. Methods The bone marrow chromosome preparation and G banding pattern for mouse were applied.Results Of the 50 G banding cells from 20 mice,10 cells were selected for banding analysis. The chromosomes were distinguished by characteristic banding patterns. The relative length and standard deviation were depicted. Some discerned points between similar banding of chromosomes were presented, such as 1, 6 and X, 4 and 5, 9, 13 and 14. In addition, 1200 metaphase cells were analysed by conventional Giemas stain. The results showed that break was 0.33%, acentric aberration and translocation were 0.08%. The spon taneous aberration frequencies were very low. Conclusion The discernment of G banding pattern of a new inbred line mouse IRM 2 is for providing scientific basis to study on abnormal structure, radiation effect, cancer research and gene map.