改良法建立妊娠期高血压疾病大鼠模型

目的寻找理想的妊娠期高血压疾病动物模型。方法60只雌性Wistar大鼠分为六组,分别为正常妊娠组（A组）、左旋亚硝酸精氨酸甲酯（L-NAME）腹腔注射组（B组）、生理盐水注射组（C组）、假手术组（D组）、双侧子宫动脉结扎术组（E组）以及L-NAME腹腔注射联合腹主动脉缩窄术组（F组）,对大鼠血压、尿蛋白值以及胎鼠、胎盘、胎鼠头的重量进行监测并观察肾脏、胎盘的病理改变。结果F组孕鼠于孕13d即出现了蛋白尿、血压升高,较B组及E组出现时间早（P〈0.05）,其胎鼠体重、胎头重量下降较各组更明显（P〈0.05）,肾小球小动脉管壁增厚、管腔狭窄;胎盘出现血管间膜增厚、纤维蛋白沉积等妊娠高血压疾病的典型病理改变。结论L-NAME腹腔注射联合腹主动脉缩窄术是更为理想的建立妊娠高血压疾病动物模型的方法。

An Improved Method to Establish Animal Model of Hypertensive Disorder Complicating Pregnancy

Objective To establish an animal model of hypertensive disorder complicating pregnancy（HDCP）with an improved method.Methods Adult female Wistar rats were randomly divided into 6 groups：normal pregnant group（group A）,intraperitoneally infected with L-NAME group（group B）,intraperitoneally injected with saline solution group（group C）,bilateral uterine artery ligation group（group D）,shame operation group（group E）and coarctation of the abdominal aorta combined with intraperitoneally infected with L-NAME（group F）.The change of systolic pressure,urine,pathological lesions in the kidney and placenta and the weight of placenta,fetal body and head were monitored.Results The treatment in group F resulted in a significant increase of maternal systolic pressure and proteinuria just within 13 days of gestation.The symptoms occurred earlier than those of the group B and E（P〈0.05）.It also caused a substantial decrease in the weight of the pups and the fetal head,accompanied by typical renal and placental pathological changes.These signs are similar to those of HDCP.Conclusion Coarctation of abdominal aorta combined with intra-peritoneal infection with L-NAME is an ideal way to establish a rat model of hypertensive disorder complicating pregnancy.