四氯化碳诱导的小鼠肝纤维化模型中SDF-1/αCXCR4的表达及其意义

目的检测四氯化碳（CCl4）诱导的肝纤维化小鼠模型肝脏SDF-1/αCXCR4的表达,评价SDF-1/αCXCR4轴与肝纤维化的关系,为研究肝纤维化肝损伤发生及损伤修复机制研究提供基础。方法选用6周龄雌性纯系C57小鼠,采用40%的CCl4/橄榄油溶液腹腔注射,剂量为1 mL/kg,每周2次,共4周,制成肝纤维化模型,取肝纤维化及正常对照组小鼠肝脏标本,采用RT-PCR及免疫组化检测SDF-1α的表达,采用RT-PCR及Western检测CXCR4受体的表达。结果与对照组相比,SDF-1α及CXCR4在肝纤维化模型小鼠肝脏组织中的表达较对照组明显上调,差异具有统计学意义（P〈0.05）。结论肝纤维小鼠肝组织的SDF-1/αCXCR4受体表达上调,为研究肝纤维化肝损伤机制及干细胞移植治疗提供理论基础。

Expression of Stromal Cell-Derived Factor 1α (SDF-1α) and CXC Chemokine Receptor 4 (CXCR4) and Their Significance in CCl4-Induced Liver Fibrosis in Mice

Objective To detect the gene expression of SDF-1α/CXCR4 in the mouse liver with fibrosis induced by CCl_4,to evaluate the association between SDF-1α/CXCR4 axis and liver fibrosis,and to promote further researches.Methods Liver fibrosis was induced in six week-old female C57 mice by intraperitoneal injection of 40% CCL_4/olive solution（1 mL/kg,twice per week for 4 weeks）.The gene expression of SDF-1α and CXCR4 were detected in livers with fibrosis and normal livers.The SDF-1α was detected by RT-PCR and immunohistochemistry,and CXCR4 was detected by RT-PCR and Western-blotting. Results The expression of SDF-1α and CXCR4 was significantly increased in liver with fibrosis compared to that in normal liver（P0.05）.Conclusion This study demonstrates the up-regulated expression of SDF-1α/CXCR4 in mouse liver with fibrosis,therefore,serving the further studies on the damaging mechanism and stem cell treatment of liver fibrosis.