东方田鼠乳酸脱氢酶同工酶的研究

目的研究乳酸脱氢同工酶在3种东方田鼠不同器官的分布.方法以大鼠和小鼠作对照,用聚丙烯酰胺凝胶电泳对3个不同地区的东方田鼠(Microtusfortis)血清、红细胞、肝脏、肾脏、肺中乳酸脱氢酶(LDH)同工酶进行分析.结果东方田鼠血浆中只有LDH5,血清中湖南洞庭湖地区、宁夏青铜峡地区的东方田鼠也只有LDH5,而东北地区的东方田鼠则含有LDH1和LDH5,3个地区的东方田鼠肾脏和肺中都有5种LDH同工酶,肝脏中除东北地区的东方田鼠含5种LDH同工酶外,其余两种东方田鼠均以LDH5为主(LDH5占95%以上).结论东北地区东方田鼠乳酸脱氢酶同工酶谱与其他两地的东方田鼠不相同.

Study on Lactate Dehydrogenase Isozyme of Microtus fortis

Objective To study the distribution of the lactate dehydrogenase isoenzyme in different tissues of field voles ( Microtus fortis ). Methods The samples were studied by polyacrylamide gel electrophoresis (PAGE) and stained by sodium lactate. Results There was only LDH 5 in plasma of field voles from all three regions and in sera of field voles from Dongting region and from Ningxia Qingtongxia region. There were two kinds of LDH isoenzymes(LDH 1 and LDH 5)in sera of field voles from Dongbei region. Five kinds of LDH isoenzymes were found in kidney and lung of field voles from three regions and in liver of field voles from Dongbei region, but LDH 5 was dominated in liver of the two other field voles(more than 95%). Conclusion\ LDH isoenzymes of field voles from Dongbei region were different to some extent from the two other regions.