中药对欧鳗实验性肝病保护作用的超微结构观察

目的探讨由丹参、五味子及甘草组成的中药煎剂对实验性肝病欧鳗肝组织超微结构的影响。方法将健康组欧鳗养于清水中,三个处理组先暴露于0.12 mg/L Cu2 溶液4 d,后4 d其中一组作为肝病未治疗组不予治疗,其余两组为肝病中药治疗组,即中药煎剂治疗低剂量组(200 mg/L)和中药煎剂治疗高剂量组(400 mg/L),实验结束时,剖检并取肝脏样本,进行电镜观察。结果与健康组相比,肝病未治疗组欧鳗肝细胞线粒体肿胀,嵴断裂、减少或结构模糊,有的消失呈空泡状;粗面内质网扩张、脱粒或出现扭曲现象;滑面内质网增生、扩张呈囊泡化;池内隔离体增多,自噬体及空泡多见;部分肝细胞核皱缩,染色质边集。各治疗实验组与肝病未治疗组相比,肝组织超微结构修复非常显著。结论研究表明该中药煎剂可显著促进铜离子中毒致肝损伤欧鳗病变肝细胞结构的修复,具有良好的抗肝损伤及修复肝损伤作用。

Ultrastructural Observation on the Mechanism of protective Effect of Chinese Herbal Medicine Decoction on Experimental Hepatosis in Anguilla anguilla

Objective The purpose of this study was to determine the restoring-capacity of a Chinese herbal medicine decoction to the ultrastructure of hepatocytes in eel with liver injury induced by Cu-exposure.Methods Eels Anguilla anguilla were injured with cupric sulfate and treated with a Chinese herbal medicine decoction mainly composed of Salvia miltiorrhiza,Schisandra chinensis(Turcz.)Baill and Radix Glycyrrhizae.At the end of the trial period,fish were killed,dissected and the liver was examined by electron microscopy.Results Compared with the control fish,the hepatocytes of Cupoisoned control fish showed mitochondria swelled with disintegration or disappearance of cristae,dilation,degranulation and disintegration of rough endoplasmic reticulum.Intracisternal sequestration,autophagosomes and vacuoles were often observed.Some nuclei of hepatocytes presented nuclear crenation.Compared with the Cu-poisoned control fish,the ultrastructure of hepatocytes of drug-treated fish showed recovery notably.Conclusion Our findings demonstrated that this Chinese herbal medicine decoction may promote the recovery of ultrastructure of hepatocytes in the copper-damaged eel liver,indicating an effective protective effect against those damages.