| 碳水化合物对牛体外受精胚胎体外发育的影响 |
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| 引用本文： | 刘东军,旭日干.碳水化合物对牛体外受精胚胎体外发育的影响[J].中国实验动物学报,2003,11(1):38-41. |
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| 作者姓名： | 刘东军 旭日干 |
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| 作者单位： | 内蒙古大学实验动物研究中心,呼和浩特,010021 |
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| 基金项目： | 内蒙古自然科学基金项目资助课题 ( 9910 4- 2 ) |
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| 摘 要： | 目的 在SOF +PVA(合成输卵管液 +聚乙烯醇 )这一化学成分明确培养系统条件下 ,观察了葡萄糖、丙酮酸和乳酸三种碳水化合物对牛体外受精胚胎体外发育的影响 ,以便为今后进一步探讨影响牛早期胚胎体外发育的因素提供实验依据。方法 牛卵母细胞体外成熟和体外受精后 ,在化学成分明确培养系统内进行体外发育培养。结果 实验 1将牛体外受精卵培养于不含有葡萄糖的SOF +PVA培养系统中 ,培养 12 0h后分别移入含有 0、1 5 0、3 30、5 0 0mmol L的SOF +PVA培养系统中 ,对照组胚胎一直在含有 1 5 0mmol L葡萄糖的SOF +PVA中培养 ,结果囊胚的发育率分别为 9 2 % a、12 1%、19 2 % b、18 9%和 11 7% (a
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| 关 键 词： | 碳水化合物 受精 体外 胚胎 牛 发育生物学 |
| 文章编号： | 1005-4847(2003)01-0038-04 |
| 修稿时间： | 2002年3月25日 |
Effects of Carbohydrates on in vitro Development of Bovine Embryos Produced in vitro |
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| LIU Dong-jun,XU Ri|gan.Effects of Carbohydrates on in vitro Development of Bovine Embryos Produced in vitro[J].Acta Laboratorium Animalis Scientia Sinica,2003,11(1):38-41. |
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| Authors: | LIU Dong-jun XU Ri|gan |
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| Abstract: | Objective The effects of glucose, pyruvate and lactate on in vitro development of bovine embryos produced in vitro in chemical defined culture system (SOF+PVA) were observed in this research. Method Bovine oocytes were matured and fertilized in vitro, then the fertilized ova were cultured in vitro in chemical defined culture medium. Results Experiment 1: Bovine IVF embryos were cultured in SOF+PVA that was not supplemented with glucose, then were cultured in SOF+PVA supplemented with 0, 1.50, 3.30 and 5.00mmol/L glucose respectively after being cultured 120 h, the embryos in control group were cultured in SOF+PVA supplemented with 1.50 mmol/L glucose during all the culture time. The results showed that the development rates of blastocysts were 9.2% a, 12.1%, 19.2% b, 18.9% and 11.7% respectively (a
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| Keywords: | Carbohydrates Fertilization in vitro Embryo Cattle Development Biology |
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