| 树鼩微卫星DNA的多态性研究 |
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| 引用本文： | 何保丽,沈培清,陈丽玲,角建林,刘汝文,李波,郑红,李玛琳.树鼩微卫星DNA的多态性研究[J].中国实验动物学报,2009,17(2):143-145. |
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| 作者姓名： | 何保丽 沈培清 陈丽玲 角建林 刘汝文 李波 郑红 李玛琳 |
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| 作者单位： | 1. 昆明医学院实验动物中心,昆明,650031
2. 昆明医学院云南省天然药物药理重点实验室,昆明,650031 |
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| 基金项目： | 云南省科技基础条件平台建设计划项目 |
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| 摘 要： | 目的探索并建立一种检测树鼩群体遗传多样性的方法。方法利用聚合酶链反应（PCR）扩增技术对30只树鼩个体的11个微卫星位点进行了遗传检测。结果所选的11个微卫星DNA位点中,有9个具有高度多态性,2个微卫星DNA位点多态性较差。结论所研究的树鼩微卫星位点中,有9个符合遗传标记特点,可用于检测树鼩群体的遗传多样性。
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| 关 键 词： | 树鼩 微卫星DNA 多态性 |
Polymorphism Microsatellites in Tree Shrews (Tupaia Belangeri Chinensis) |
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| HE Bao-li,SHEN Pei-qing,CHEN Li-ling,JIAO Jian-lin,LIU Ru-wen,LI Bo,ZHENG Hong,LI Ma-lin.Polymorphism Microsatellites in Tree Shrews (Tupaia Belangeri Chinensis)[J].Acta Laboratorium Animalis Scientia Sinica,2009,17(2):143-145. |
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| Authors: | HE Bao-li SHEN Pei-qing CHEN Li-ling JIAO Jian-lin LIU Ru-wen LI Bo ZHENG Hong LI Ma-lin |
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| Institution: | 1.Laboratory Animal Center,Kunming Medical College,Kunming 650031,China;2.Yunnan Key Laboratory of Pharmacology for Natural Product Research,Kunming 650031) |
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| Abstract: | Objective In order to explore and set up an accurate and convenient method for genetic assessment of tree shrews.Method DNA polymorphic microsatellites of Tupaia Belangeri chinensis were analyzed using PCR technique in 30 tree shrews.Results Nine polymorphic microsatellites loci,2 non-polymorphic microsatellites loci were screened.Conclusion Nine of eleven DNA polymorphic microsatellites 1oci can be used for genetic assessment of tree threw. |
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| Keywords: | Tree threw Microsatellites DNA Polymorphism |
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