| 猴B病毒PCR检测方法的建立 |
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| 引用本文： | 李华,代解杰,瞿素,刘国栋,胡凝珠.猴B病毒PCR检测方法的建立[J].中国实验动物学报,2005,13(1):48-50. |
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| 作者姓名： | 李华 代解杰 瞿素 刘国栋 胡凝珠 |
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| 作者单位： | 中国医学科学院,协和医科大学,医学生物学研究所,昆明,650118 |
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| 摘 要： | 目的 建立检测猴血B病毒的PCR方法。方法 根据MakotoH报道的引物 ,用PCR方法直接扩增猴血B病毒及扩增经Vero细胞培养后的猴血B病毒 ,扩增产物连于pGEM T载体。结果 这四对引物可同时对猴血B病毒及经Vero细胞培养后的猴血B病毒进行扩增 ,扩增结果一致 ,对扩增片段克隆测序的结果证实 ,其与美国猴B病毒E2 4 90株同源性为 10 0 %。结论 建立了从血样中直接检测猴B病毒DNA的PCR方法。
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| 关 键 词： | 疱疹病毒Ⅰ型 猕猴 聚合酶链反应 |
| 文章编号： | 1005-4847(2005)01-0048-03 |
| 修稿时间： | 2004年4月6日 |
Establishment of PCR Method for Detection of Monkey B Virus |
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| LI Hua,DAI Jie-jie,QU Su,LIU Guo-dong,HU Ning-zhu.Establishment of PCR Method for Detection of Monkey B Virus[J].Acta Laboratorium Animalis Scientia Sinica,2005,13(1):48-50. |
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| Authors: | LI Hua DAI Jie-jie QU Su LIU Guo-dong HU Ning-zhu |
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| Abstract: | Objective To establish a PCR method to dete4ct monkey B virus (BV). Method According to published primers provided by Makoto H, w e used PCR method to amplify BV DNA directly from monkey blood or indirectly from propagated bloo d BV cultured in Vero cells. PCR segments were bound to pGEM-T vector.R esults The primers were able to amplify both blood BV and propagated bl ood BV and give identical results. The amplified segment was sequenced and was 1 00% homologous with E2490 (American BV strain). Conclusion A PC R method has been established to directly detect monkey B virus from blood. |
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| Keywords: | Herpesvirus 1 cercopithecine Polymerase cha in reaction |
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