西藏小型猪H-FABP基因的PCR-RFLP研究

目的研究西藏小型猪心脏脂肪酸结合蛋白（H-FABP）基因5’-上游区和第二内含子内的遗传变异。方法应用PCR-RFLP技术测定30头西藏小型猪H-FABP的基因型。结果（1）在5’-上游区的Hinf I-RFLP位点上，西藏小型猪表现出多态性，等位基因h的频率为0．80；（2）在在第二内含子内的Hae Ⅲ-RFLP位点上，西藏小型猪均为DD纯合子；（3）在第二内含子内的Hinf I＊-RFLP位点上，除一头猪表现为bb基因型外，其余猪都表现为BB基因型，等位基因B的频率为0．97；（4）除Hae Ⅲ-RFLP位点外，在其余位点上西藏小型猪均处于Hardy-weinberg不平衡状态。（5）在Hinf I-RFLP中，西藏小型猪表现为中度多态性（0．25〈PIC〈0．5），而在其他位点的RFLP中表现为低度多态（PIC〈0．25）。结论可以利用西藏小型猪H-FABP基因5’-上游区的多态遗传标记来分析其与肌内脂肪的关系。

Polymorphism of Heart Fatty Acid-Binding Protein Gene in Tibet Minipig Populations Detected by PCR-RFLP

Objective To study the genetic variation in 5＇-upstream and the second intron of heart fatty acid-binding protein （H-FABP） gene in Tibet minipigs. Methods PCR-restriction fragment length polymorphism （PCR-RFLP） assay was used to measure the H-FABP genotype in 30 Tibet minipigs. Result （ 1 ） The Tibet minipigs had polymorphism at the Hinf I-RFLP site of the 5＇-upstream region, and the frequency of h allele in Tibet minipigs was 0.80. （2） The Tibet minipigs presented only DD genotype at the Hae Ⅲ-RFLP site; （3） At the Hinf I＊-RFLP site of the second intron, all the Tibet minipigs presented BB genotype except only one minipig presented bb genotype, and the frequency of B allele was 0.97; （4） Other than at the Hae Ⅲ-RFLP site, all the Tibet minipigs populations were not in Hardy-Weinberg equilibrium. （5） The genetic diversity in Tibet minipigs was at a moderate level （0.25 〈 PIC 〈 0.5） at the HinfI-RFL P site while in other sites, the level was low （ PIC 〈 0.25）. Conclusion Genetic polymorphism in 5＇-upstream region of H-FABP gene in Tibet minipigs can be used as a marker for analysis on its relationship with intramuscular fat.