实验性糖尿病小鼠的血清氨基酸代谢谱

目的通过测定2型糖尿病小鼠血清氨基酸代谢谱的变化,探讨代谢轮廓分析结合模式识别技术在糖尿病动物模型中的应用。方法 SPF级雄KM小鼠高脂饲料喂养4周后,腹腔注射链脲菌素(streptozotocin,STZ)建立2型糖尿病模型,动态监测空腹血糖(FBG)变化,分别于造模后第4周处死,收集小鼠血清,检测甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)水平。采用高效液相色谱(HPLC)柱前衍生方法检测小鼠血清中氨基酸代谢谱的变化。结果 2型糖尿病小鼠FBG、TG、TC明显升高,差异均有显著性。利用代谢轮廓分析可以对模型组大鼠代谢谱与对照组完全区分。结论小鼠成模后体内氨基酸发生了明显变化。从差异变量中鉴定出4个氨基酸对组间贡献较大(精氨酸、苯丙氨酸、赖氨酸、牛磺酸)。氨基酸的代谢轮廓分析结合模式识别技术可以在一定程度上反映2型糖尿病小鼠的代谢变化。

Serum amino acid profiling in experimental diabetic mice

Objective To investigate the variation of serum amino acids in diabetic mice in order to explore the application of metabolic profiling analysis in research of diabetes mellitus in mice. Methods Healthy male adult SPF Kunming mice were fed with high-fat diet for 4 weeks and then injected with streptozotocin （STZ）. Fasting blood glucose （FBG） was monitored weekly after STZ injection. Blood samples of normal mice and model mice were collected at 4 weeks after STZ injection. The metabolic profiles were analyzed using pre-column derivatization HPLC. Results Compared with the control mice,the FBG, TG, and FFA were significantly higher than those in the model mice. The metabolic profile of the model mice was significantly different from that of the control mice. Conclnsions Pattern recognition method is ap- plied and demonstrates that four amino acid markers （arginine, phenylalanine, lysine, tuarine） are different between the normal and model mice. Amino acid metabolism profiling combined with pattern recognition may reflect to a certain extent the pathological changes of type 2 diabetic mice.