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三种不同冷冻保护剂对小鼠附睾冷冻效果的比较
引用本文:李淼,郁丽丽,张艺宝,强苏静,刘丽均,徐平.三种不同冷冻保护剂对小鼠附睾冷冻效果的比较[J].中国实验动物学报,2014(2):62-65,I0007.
作者姓名:李淼  郁丽丽  张艺宝  强苏静  刘丽均  徐平
作者单位:[1]中国科学院上海生命科学研究院,上海201615 [2]上海斯莱克实验动物有限责任公司,上海201615 [3]南京农业大学动物医学系,南京210095
基金项目:上海市科研计划项目(111409022700);国家科技支撑计划课题(2013BAK11B02).
摘    要:目的探讨三种不同冷冻保护剂对C57BL/6J小鼠附睾冷冻的效果。方法性成熟并交配过的6~8周龄C57BL/6J雄鼠附睾,分别用3种不同冷冻保护剂二甲基亚砜(DMSO)、丙二醇(PROH)、R18S3进行玻璃化冷冻、复苏和精子采集,观察比较三个实验组的复苏精子形态、存活率以及生殖能力。结果三组冷冻复苏分离后的精子形态完整,具有镰刀头状结构;荧光染色后PROH组精子存活率为(88.17±3.43)%,明显高于DMSO组:(61.17±10.65)%和R18S3组(16.83±6.49)%(P0.05);经辅助体外受精后均具有受精能力。获得的胚胎移植后可得到正常子代小鼠(DMSO∶PROH∶R18S3=13∶8∶17)。结论 3种冷冻保护剂均适用于C57BL/6J小鼠附睾冷冻,但PROH冷冻效果较好。

关 键 词:小鼠附睾  冷冻保护剂  冷冻效果

Comparison of the effect of three cryoprotectants on vitrification-cryopreservation of mouse epididymis
LI Miao,YU Li-li,ZHANG Yi-bao,QIANG Su-jing,LIU Li-jun,XU Ping.Comparison of the effect of three cryoprotectants on vitrification-cryopreservation of mouse epididymis[J].Acta Laboratorium Animalis Scientia Sinica,2014(2):62-65,I0007.
Authors:LI Miao  YU Li-li  ZHANG Yi-bao  QIANG Su-jing  LIU Li-jun  XU Ping
Institution:1. Shanghai Institute for Biological Sciences, Chinese Academy of Sciences,Shanghai 201615, China; 2. Shanghai SLAC Laboratory Animal Ltd. Co. ,Shanghai 201615; 3. Veterinary College of Nanjing Agriculture University, Nanjing 210095)
Abstract:Objective To compare the effect of three cryoprotectants on vitrification-cryopreservation of C57BL/6J mouse epididymis.Method Epididymises from 6-8-week old male C57BL/6J mice (age) were cryopreserved using DMSO, PROH and R18S3 cryoprotectant solution and thawed , respectively.The morphology of sperm from thawed epididy-mis, the rate of post-thawing survival and reproductive capacity were determined to evaluate the freezing efficiency of the three cryoprotectants .Results The sperms from thawed epididymis of the three groups were all well-preserved structural-ly.The survival rate of sperms was 88.17%, 61.17% and 16.83% in the PROH, DMSO and R18S3 cryoprotectant solu-tions, respectively, and there were significant differences between the three cryopreservation groups (P〈0.05 for all). The number of pups from the DMSO , PROH and R18S3 groups were 13, 8 and 17 mice after ICSI and embryo implantation , respectively.Conclusions All the three cryoprotectants DMSO , PROH and R18S3 solutions are suitable for vitrification-cryopreservation of C57BL/6J mouse epididymis.But PROH is the preference of these cryoprotectant solutions .
Keywords:Mouse  Epididymis  Cryoprotectant solution  Freezing efficiency  Vitrification
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