BALB/c小鼠子宫内膜异位症痛经模型的建立

目的建立近交系BALB/c小鼠子宫内膜异位症痛经模型。方法采用自体移植法将小鼠自体子宫组织块移植到腹膜,复制子宫内膜异位症模型,将术后的小鼠随机分为4组,手术+雌激素+缩宫素组、手术+雌激素组、手术+缩宫素组、手术组,并设假手术组和雌激素+缩宫素组,术后1~12 d采用不同方案诱发小鼠扭体反应,记录扭体潜伏期及扭体次数,并取异位灶行HE染色和病理组织学观察,筛选建立内异症痛经模型的最佳方案。结果除假手术组、雌激素+缩宫素组外,各组小鼠移植物均生长良好,镜下可见子宫内膜腺体及间质细胞,证实内异症造模成功,与手术+缩宫素组、手术组相比,手术+雌激素+缩宫素组、手术+雌激素组移植物体积明显增大,差异有显著性(P0.01);手术+雌激素+缩宫素组扭体发生率100%,雌激素+缩宫素组扭体发生率80%,手术+缩宫素组扭体发生率50%,其余组未出现扭体反应,组间差异有显著性(P0.01);与手术+缩宫素组、雌激素+缩宫素组相比,手术+雌激素+缩宫素组扭体潜伏期明显缩短(P0.01,P0.05),扭体次数明显增多(P0.01,P0.05),差异有显著性。结论手术+雌激素+缩宫素组为建立内异症痛经模型的最佳方案,方法简单易行,可用于内异症痛经发病机制及药物治疗研究。

Establishment of a mouse model of endometriosis dysmenorrhea

Objective To establish a BALB/c mouse model of endometriosis dysmenorrhea. Methods Auto-transplantation of uterine tissue into the peritoneum was applied to generate endometriosis model in 60 healthy, 6-8-week old female BALB/c mice. The mice were divided into 4 groups： operation＋estrogen＋oxytocin group, operation＋estrogen group, operation＋ oxytocin group, and operation group. Besides, an estrogen＋oxytocin group and sham operation group were also set up. Different regimens were applied to treat the mice from 1st to 12th day after operation, observed the writhing response, and collected tissue samples from the ectopic foci to examine the histopathological characteristics in order to screen the best regimen. Results Except for the estrogen＋oxytocin group and sham operation group, the ectopic foci of all the other groups developed well. The lesion volumes of the operation＋estrogen＋oxytocin group and operation＋ estrogen group were significantly larger than that in the other groups（P〈0.01）. The incidence of writhing response of the operation＋estrogen＋oxytocin group was 100%, and there were statistically significant differences among different groups （P〈0.01）. The writhing latency and writhing frequency of the operation＋estrogen＋oxytocin group were significantly different from that in the other groups （P〈0.01 or P〈0.05）. Conclusions Operation＋estrogen＋ oxytocin is the best method to establish a mouse model of endometriosis dysmenorrhea, and is simple to perform. This animal model can be used to investigate the mechanisms and treatment of endometriosis dysmenorrhea.