| GFP在绿色荧光裸鼠不同组织中的表达及分析 |
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| 引用本文： | 尤金炜,董敏,刘彪,梁磊,田迎,胡文娟,田小芸,方天,周森妹,赵志刚,恽时锋.GFP在绿色荧光裸鼠不同组织中的表达及分析[J].中国实验动物学报,2014(5):67-70. |
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| 作者姓名： | 尤金炜 董敏 刘彪 梁磊 田迎 胡文娟 田小芸 方天 周森妹 赵志刚 恽时锋 |
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| 作者单位： | 南京军区南京总医院比较医学科，全军实验动物科普与伦理教育基地，全国科普教育基地，南京 210002 |
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| 基金项目： | 南京军区南京总医院课题(2012052)。 |
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| 摘 要： | 目的研究外源绿色荧光蛋白（green fluorescent protein,简称GFP）基因在BALB/c绿色荧光裸鼠主要器官组织中的表达及其差异。方法小动物成像系统和RT-PCR方法检测GFP的组织分布以及荧光表达水平情况。结果经活体荧光影像系统观察及PCR方法检测发现GFP可以在裸鼠多个器官组织中表达,其中在胰腺、心脏、全脑、皮肤、睾丸中表达量较高。结论外源绿色荧光蛋白可以在模型动物体内成功表达且稳定遗传,其中在胰腺组织中高表达。
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| 关 键 词： | 绿色荧光蛋白 裸鼠 组织分布 |
Distribution of green fluorescent protein (GFP) expression in green fluorescent nude mice |
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| YOU Jin-wei,DONG Min,LIU Biao,LIANG Lei,TIAN Ying,HU Wen-juan,TIAN Xiao-yun,FANG Tian,ZHOU Sen-mei,ZHAO Zhi-gang,YUN Shi-feng.Distribution of green fluorescent protein (GFP) expression in green fluorescent nude mice[J].Acta Laboratorium Animalis Scientia Sinica,2014(5):67-70. |
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| Authors: | YOU Jin-wei DONG Min LIU Biao LIANG Lei TIAN Ying HU Wen-juan TIAN Xiao-yun FANG Tian ZHOU Sen-mei ZHAO Zhi-gang YUN Shi-feng |
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| Institution: | ( Department of Comparative Medicine, Nanjing General Hospital of Nanjing Military Command, Army Education Base of Science and Ethics of Experimental Animals, National Science Education Base, Nanjing 210002, China) |
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| Abstract: | Objective To study whether the green fluorescent protein （ GFP） gene can be successfully expressed in green fluorescent nude mice and the tissue distribution characteristics.Methods Small animal imaging system and RT-PCR assay were used to detect the GFP tissue distribution and fluorescence expression level.Results The GFP can be expressed in multiple tissues in green fluorescent nude mice.A higher expression was observed in the pancreas, heart, brain, and skin.Conclusion Exogenous GFP can be stably expressed and inherited in green fluorescent nude mice, with the highest expression in the pancreas. |
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| Keywords: | Green fluorescent protein ( GFP) Nude mice Tissue distribution |
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