不同剂量链脲菌素对恒河猴某些常规生理指标的影响

目的　研究链脲菌素 (STZ)对恒河猴摄食、饮水、排尿、体重、血糖及尿糖等生理指标的影响 ,为建立糖尿病动物模型积累资料。方法　通过静脉给 7只恒河猴注射不同剂量的STZ。结果　使用不同剂量STZ后 ,7只恒河猴均在不同时间、不同程度出现与人糖尿病相类似的“三多一少”症状 ,同时对糖尿病猴使用不同剂量胰岛素可使其症状减轻。尤其是中、高剂量组的动物症状较为明显 ,而低剂量组体重有一个短时间的增加后又迅速下降。实验组中血糖和尿糖均出现不同程度的升高 ,以中、高 2个剂量组的动物变化较大。结论　采用中、高剂量组的STZ可诱导类似人类糖尿病的急性动物模型 ,而使用胰岛素治疗或低剂量STZ可使该动物模型疾病病程延长 ,有利于进行其并发症的研究。

Effects of Different Dosage of Streptozotocin on Some Physiological Data in Rhesus Monkey

Objective To study physiological data of food and water intake, urination, body weight, plasma glucose and glycosuria with streptozotocin(STZ) in rhesus monkeys, which was essential to establish animal model for diabetes mellitus(DM). Methods Seven rhesus monkeys were injected intravenously with different doses of STZ. Results The seven monkeys showed the same clinical features as those of diabetes mellitus patients at a different time and extent, such as polyphagia, polydipsia, polyuria, hyperglycemia, hyperglycosuria, body weight loss. The clinical characteristics were lightened and controlled by using insulin. Especially the clinical symptoms of mid-dose and high-dose of STZ were more obvious than those of low-dose in monkeys. The body weight of low-dose monkeys rose in a short time, then dropped rapidly. Plasma glucose and glycosuria of monkeys were increased with STZ injected, especially mid-dose and high--dose monkeys varied obviously. Conclusion The acute animal model similar to human DM was induced by mid-dose and high-dose of STZ injected. The course of DM animal model may be extended by using insulin treatment or low-dose of STZ, which is suitable for studying complications of DM.