| 奖励性操作式条件反射任务在大鼠学习记忆研究中的应用 |
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| 引用本文： | 石哲,陈善广,陈玲玲,孙秀萍,宋广青,王琼,常琪,李海清,李莹辉,曲丽娜,刘新民.奖励性操作式条件反射任务在大鼠学习记忆研究中的应用[J].中国实验动物学报,2012,20(4):9-15. |
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| 作者姓名： | 石哲 陈善广 陈玲玲 孙秀萍 宋广青 王琼 常琪 李海清 李莹辉 曲丽娜 刘新民 |
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| 作者单位： | [1]中国医学科学院北京协和医学院药用植物研究所药理毒理中心,北京100193 [2]中国航天员科研训练中心,北京100193 [3]湖南中医药大学,湖南长沙410208 [4]北京鑫海华仪科技有限公司,北京100193 |
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| 基金项目： | 国家自然科学基金面上项目（30973888）;国家科技重大专项新药创制专项：（2009ZX09502-014）;国家航天医学与基础应用重点实验室开放课题（SMFAl2805）. |
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| 摘 要： | 目的将奖励性操作式条件反射方法应用于学习记忆研究。方法首次采用奖励性操作式条件反射方法,设置单次操作训练、连续多次操作训练、信号辨识与消退四种检测模式,研究Wistar大鼠和SHR大鼠学习记忆能力。结果奖赏训练中,SHR组鼻触次数显著增多（vs.Wistar＆Donepezil,P〈0.05）;单次操作训练中,三组动物对操作反应的获得无显著差异;连续多次操作学习任务中,SHR组错误操作次数增多（vs.Wistar,P〈0.05）,奖赏获得次数减少（vs.Donepezil,P〈0.001）,反应准确率显著降低（vs.Wistar＆Donepezil,P〈0.05）;信号辨识任务中SHR组错误反应次数显著增多（vs.Wistar＆Donepezil,P〈0.05）,而反应准确率降低,组间差异有显著性（vs.Wistar＆Donepezil,P〈0.05）;消退实验中,三组动物差异无显著性。神经递质检测发现,SHR组大鼠谷氨酸（1.0639±0.07086）mg/g]、乙酰胆碱（2.7760±0.2609）μg/g]与五羟色胺（1.2200±0.1137）μg/g]含量均显著低于Wistar组大鼠（P〈0.05）,多奈哌齐组大鼠乙酰胆碱含量显著增加（3.9344±0.2747）μg/g]（vs.Wistar,P〈0.05;vs.SHR,P〈0.001）。结论奖励性操作式条件反射方法可作为一种正性增强条件反射检测新方法应用于大鼠学习记忆研究。
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| 关 键 词： | 操作式条件反射 奖赏 Wistar大鼠 SHR大鼠 学习记忆 |
Evaluation of reward-relevant learning and memory behavior with operant conditioning task in rats |
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| SHI Zhe,CHEN Shan-guang,CHEN Ling-ling,SUN Xiu-ping,SONG Guang-qing,WANG Qiong,CHANG Qi,LI Hai-qing,LI Ying-hui,QU Li-na,LIU Xin-min.Evaluation of reward-relevant learning and memory behavior with operant conditioning task in rats[J].Acta Laboratorium Animalis Scientia Sinica,2012,20(4):9-15. |
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| Authors: | SHI Zhe CHEN Shan-guang CHEN Ling-ling SUN Xiu-ping SONG Guang-qing WANG Qiong CHANG Qi LI Hai-qing LI Ying-hui QU Li-na LIU Xin-min |
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| Institution: | 1(1.Research Center for Pharmacology ＆ Toxicology,Institute of Medicinal Plant Development(IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China; 2.Astronaut Center of China,Beijing,100193;3.Hunan University of Traditional Chinese Medicine, Changsha,Hunan 410208;4.Beijing Xin-hai Hua-yi Technology Limited Company,Beijing,100193)SHI Zhe1,CHEN Shan-guang2,CHEN Ling-ling 1,3,SUN Xiu-ping1,SONG Guang-qing1,WANG Qiong1,2, CHANG Qi1,LI Hai-qing4,LI Ying-hui2,QU Li-na2,LIU Xin-min1(1.Research Center for Pharmacology ＆ Toxicology,Institute of Medicinal Plant Development(IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China; 2.Astronaut Center of China,Beijing,100193;3.Hunan University of Traditional Chinese Medicine, Changsha,Hunan 410208;4.Beijing Xin-hai Hua-yi Technology Limited Company,Beijing,100193) |
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| Abstract: | Objective To apply reward directed operant conditioning task for learning and memory research in laboratory animals.Methods Sixteen adult male SHR rats and 8 Wistar rats were included in this study.Operant conditioning procedure was used to assess the learning ability of Wistar and SHR rats with magazine training,fixed ratio,signal discrimination and extinction procedure.Results Significant difference was found in magazine training.The SHR rats performed more NP vs.Wistar rats and SHR rats treated with donepezil（P〈0.05）.There was no significant difference during FR1 schedule.While in higher response ratio FR2 and FR4 schedule,the SHR group showed significantly higher error responses（vs.Wistar rat group,P〈0.05）,lower rewards eared（vs.Donepezil-treated SHR rat group,P〈0.001）,and significantly lower correct accuracy rate（vs.Wistar rats and Donepezil-treated SHR rats,P〈0.05）.Significantly higher error responses were found during signal discrimination task（vs.Wistar rats and Donepezil-treated SHR rats,P 〈0.05）,and lower correct accuracy rate（vs.Wistar rats and Donepezil-treated SHR rats,P〈0.05）.No significant difference was found during extinction.Neurotransmitter test showed that the hippocampal content of glutamate （1.0639±0.07086） mg/g],acetylcholine （2.7760±0.2609）μg/g] and 5-HT （1.2200±0.1137）μg/g] were significantly lower in the SHR group（vs.Wistar rat group,P〈0.05）.The content of acetylcholine （3.9344±0.2747）μg/g] was higher in the Donepezil-treated SHR rats group（vs.Wistar rats,P〈0.05;vs.SHR rats,P〈0.001）.Conclusions Reward directed operant conditioning is a type of positive reinforced conditioning.This task can be used as a new behavior test method for learning and memory study. |
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| Keywords: | Operant conditioning Reward Wistar rats SHR rats Learning Memory |
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