| 人CD55基因的克隆及其转基因构件的组装 |
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| 引用本文： | 陈莹,黄惠民,刘锦纷,丁文祥,苏肇伉,王建荣.人CD55基因的克隆及其转基因构件的组装[J].中国实验动物学报,2000,8(1):16-21. |
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| 作者姓名： | 陈莹 黄惠民 刘锦纷 丁文祥 苏肇伉 王建荣 |
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| 作者单位： | 1. 上海第二医科大学附属新华医院儿胸外科,上海,200092
2. 上海市农科院畜牧兽医研究所转基因动物实验室,201106 |
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| 摘 要： | 本实验采用人肝组织作为RNA的来源 ,经RT -PCR扩增得到CD55基因的cDNA片段。与人α -珠蛋白启动子及其polyA序列重组 ,插入质粒载体pGEM - 5zf,获得了可用于受精卵原核显微注射的基因构件 ,为建立人CD55转基因动物模型奠定了基础。
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| 关 键 词： | CD55基因 克隆 转基因构件 |
Clonig of Human CD55 Gene and Recombing Its Transgenic Constructs |
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| Chen-Ying,Hiang Hui-min,Liu Jin-fen,Ding Wen-xiang,Su Zhao-kang,Wang Jian-rong.Clonig of Human CD55 Gene and Recombing Its Transgenic Constructs[J].Acta Laboratorium Animalis Scientia Sinica,2000,8(1):16-21. |
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| Authors: | Chen-Ying Hiang Hui-min Liu Jin-fen Ding Wen-xiang Su Zhao-kang Wang Jian-rong |
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| Abstract: | First,we use human hepatic RNA as templ ate and got CD55
cDNA by PT-PCR. Secondly, CD55 cDNA is combined with promoter a nd polyA of human α-globin, then inserted into
plasmid pGEM-5zf. At last,we got a transgenic constructs' of pGEM-5zf-α-globin-CD55 cDNA'. The constructs
ma y use to found human CD55 transgenic animal model. |
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| Keywords: | CD55 gene Cloning Transgenic constructs |
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