左归丸含药血清对成骨细胞IL-1、IL-6和COX-2表达的影响

目的通过观察左归丸含药血清对成骨细胞白细胞介素-1(IL-1)、白细胞介素-6(IL-6)和环加氧酶-2(COX-2)表达的影响,探讨其治疗骨质疏松症的作用机制。方法体外分离、培养成骨细胞,实验分为3组:正常血清组、卵巢切除(OVX)血清组和OVX含药血清组。采用免疫组化法,检测成骨细胞IL-1、IL-6和COX-2的表达。结果OVX血清组成骨细胞IL-1、IL-6和COX-2的表达明显强于正常血清组,而OVX含药血清组的表达较OVX血清组明显减弱,与正常血清组比较,则无显著性差异。结论在去势状态下,左归丸可能是通过抑制成骨细胞IL-1、IL-6和前列腺素E2(PGE2)的分泌,进而达到治疗骨质疏松的作用。

Effects of Serum Containing Zuogui Pill Substances on the Expression of IL-1, IL-6 and COX-2 in Osteoblasts in vitro

Objective To investigate the effects of serum containing substances of a Chinese traditional drug, Zuogui Pill, on expression of IL-1, IL-6 and COX-2 in osteoblasts, and to study the mechanism of action of Zuogui Pill treatment for osteoporosis. Methods Osteoblasts were obtained from newborn rats calvaria by collagenase-trypsin digestion. Three groups were included in the experiment: normal serum group, serum of ovariectomized (OVX) model group and serum of Zuogui pill-treated OVX model group. Immunohistochemical techniques were used to detect the expression of IL-1, IL-6 and COX-2. Results Expression of IL-1, IL-6 and COX-2 in osteoblasts in the serum of OVX model group was increased compared with that in the normal serum group, and their expression in the serum of Zuogui pill-treated OVX model group was significantly decreased as compared with those in serum of OVX model group, but did not show significant difference from those of normal serum group. Conclusion The serum containing Zuogui pill substances can inhibit the secretion of IL-1, IL-6 and PGE_2, which may be one of the mechanisms of action of Zuogui Pill treatment for osteoporosis.