Ultraviolet radiation-induced neoplastic transformation of normal human cells,in vitro |
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Authors: | GE Milo SA Weisbrode R Zimmerman JA McCloskey |
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Institution: | 1. Department of Physiological Chemistry (G.M.), Comprehensive Cancer Center, 1900 Coffey Road, Columbus, OH 43210 U.S.A.;2. Department of Veterinary Pathobiology (S.W.) Ohio State University, 1900 Coffey Road, Columbus, OH 43210 U.S.A.;3. Department of Physiology, Harvard School of Public Health, Harvard University, Boston, MA 02115 U.S.A.;4. Northrop Service, Inc., Environmental Sciences Center, P.O. Box 12313, Research Triangle Park, NC 27709 U.S.A. |
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Abstract: | Human foreskin cell cultures in scheduled DNA synthesis (S phase) of the cell cycle were exposed to UV irradiation at a dose of 10 J · m?2 in the presence of insulin. These treated cell populations, when selectively passaged in a high amino acid supplemented complete growth medium (CM) after 20 Dulbecco's phosphate buffered saline (pH 6.8) (PDL), were able to be grown in soft agar. These treated cell populations were also grown in 1% serum supplemented growth medium and at 41°C in 10% serum supplemented growth medium. Cell populations 4–5 PDL after treatment exhibited altered colony morphology and altered lectin agglutination profiles but would not grow in soft agar. These events appeared to be associated with the early stages in the expression phase of the transformed phenotype. After 20 PDL, we observed that these cells would grow in soft agar at a frequency of 20 colonies/105 cells seeded in soft agar. The cell populations derived from these colonies, when propagated and injected into the nude mice, formed myxofibromas at the injection sites rather than the type of tumor (fibrosarcoma) previously described for chemical carcinogen-induced neoplasms. |
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Keywords: | CM complete growth medium FBS fetal bovine serum MEM minimum essential medium NFS neonatal foreskins PDL Dulbecco's phosphate buffered saline (pH 6 8) RCE relative colony-forming efficiency S phase scheduled DNA synthesis |
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