Comparative painting of mammalian chromosomes |
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| Authors: | Johannes Wienberg Roscoe Stanyon |
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| Institution: | aDepartment of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK;bInstitut für Anthropologie und Humangenetik, München, Germany;cDipartimento di Scienze Antropologiche, Universitá di Genova, Via Balbi 4, 16126 Genoa, Italy |
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| Abstract: | Comparative chromosome painting has shown that synteny has been conserved for large segments of the genome in various placental mammals. Advances such as spectral karyotyping and multicolour ‘bar coding’ lend speed and precision to comparative molecular cytogenetics. Reciprocal chromosome painting and hybridisations with probes such as yeast artificial chromosomes, cosmids, and fibre fluorescence in situ hybridisation allow subchromosomal assignments of chromosome regions and can identify breakpoints of rearranged chromosomes. Advances in molecular cytogenetics can now be used to test the hypothesis that chromosome rearrangement breakpoints in human pathology and in evolution are correlated. |
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| Keywords: | Abbreviations: FACS fluorescence-activated cell sorting FISH fluorescence in situ hybridisation YAC yeast artificial chromosome |
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