| 盐酸胍诱导的α淀粉酶去折叠与再折叠 |
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| 引用本文： | 张学忠,吴晓霞,汪大伟,丁忠田.盐酸胍诱导的α淀粉酶去折叠与再折叠[J].中国生物化学与分子生物学报,1993,9(6):648-652. |
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| 作者姓名： | 张学忠 吴晓霞 汪大伟 丁忠田 |
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| 作者单位： | 吉林大学酶工程国家重点实验室，吉林大学酶工程国家重点实验室，吉林大学酶工程国家重点实验室，吉林大学酶工程国家重点实验室 长春 130023，长春 130023，长春 130023，长春 130023 |
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| 摘 要： | 利用蛋白质内源荧光和酶活性两种信号以及荧光偏振,HPLC和停流等方法研究了盐酸胍诱导的α淀粉酶去折叠与重折叠的平衡转变和动力学。实验结果表明α淀粉酶去折叠与重折叠是两个不同的过程;变性与复性过程中可能伴有聚集体生成;去折叠与重折叠均为双相过程,重折叠大约始于2秒之后。
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| 关 键 词： | α淀粉酶 盐酸胍 去折叠与再折叠 |
| 收稿时间： | 1993-12-20 |
Unfolding-Refolding Transition of α amylase Induced by Guanidine Hydrochloride |
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| Zhang,Xue-zhong Wu,Xiao-xia Wang,Da-wei Ding,Zhong-tian.Unfolding-Refolding Transition of α amylase Induced by Guanidine Hydrochloride[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(6):648-652. |
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| Authors: | Zhang Xue-zhong Wu Xiao-xia Wang Da-wei Ding Zhong-tian |
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| Institution: | (State key Laboratory of Enzyme Engineerring, Jilin University, Changchun 130023 |
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| Abstract: | The unfolding-refolding transition and the time course of a amylase induced by guanidine hydrochloride (GuHCl) have been studied with two different signals: the fluorescence and activity of the enzyme as well as by the methods of fluorescence polarization, HPLC and stopped-flow. The experimental results indicate that unfolded molecules of α-amylase refolded through different intermediates from that of unfolding. The formation of aggregate occured during unfolding and refolding. The kinetic data showed that a fast course completed in a few seconds followed by a slow course during the unfolding and refolding of the enzyme. |
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| Keywords: | α-Amylase Guanidine Hydrochloride Unfolding-refolding |
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