| DCM和DMF对DCC-HOBt系统催化酯化及酰化反应的影响 |
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| 引用本文： | 张志刚,何素婷,何明华,蔡豪斌,何水淋.DCM和DMF对DCC-HOBt系统催化酯化及酰化反应的影响[J].中国生物化学与分子生物学报,1997,13(2):177-180. |
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| 作者姓名： | 张志刚 何素婷 何明华 蔡豪斌 何水淋 |
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| 作者单位： | 桂林医学院生物工程研究所!桂林，541003 |
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| 摘 要： | DCC-HOBt系统在DCM和DMF溶剂中，催化Fmoc-Leu-OH与王氏树脂的酯化反应，其结合率分别为18％和10％．该系统催化Fmoc-Phe-OH与H2N-Leu-resin的酰化反应中，溶剂为DCM时，反应30min，其结合率为100％；当DCM：DMF体积比为1：3．5时，反应130min，其结合率为81％，表明DCC-HOBt系统宜在DCM非极性溶剂中催化肽键的形成．
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| 关 键 词： | 多肽合成 Fmoc固相合成法 酯化 酰化 |
| 收稿时间： | 1997-04-20 |
Effect of DCM and DMF on Esterification and Acylation in DCC-HOBt System |
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| Zhang Zhi-Gang,He Su-Ting,He Ming-Hua,Cai Hao-Bin,He Shui-Lin.Effect of DCM and DMF on Esterification and Acylation in DCC-HOBt System[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(2):177-180. |
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| Authors: | Zhang Zhi-Gang He Su-Ting He Ming-Hua Cai Hao-Bin He Shui-Lin |
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| Institution: | (Institute of Bioengineering. Guilin Medical College, Guilin 541003 |
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| Abstract: | The rate of the anchoring of Fmoc-Leu-OH to Wang's resin, catalyzed by DCC-HOBt activating system, is 18% in DCM and 10% in DMF, respectively. The rate of the coupling of Fmoc-Phe-OH to H2N-Leu-resin catalyzed by DCC-HOBt is 100% for 30min in DCM; 81% for 130min in a mixed solvent of DCM and DMF(v/v, 1: 3. 5). The result shows that DCM is a suitable solvent for DCC-HOBt system in peptide-bond formation. |
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| Keywords: | Peptide synthesis Fmoc-solid-phase synthesis Esterification Acylation |
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