晶状体生化的研究:正常和亚硒酸钠诱发白内障大鼠晶状体中与谷胱甘肽代谢有关的三种酶活性的测定

测定了用亚硒酸钠诱发的大鼠白内障晶状体中谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GSSG-R)和谷胱甘肽硫转移酶(GSH-S)的活性,并与正常晶休中这三种酶的活性作了比较。结果表明,核浊浑期晶状体中GSH-Px的活性比正常晶状体的高一倍,但在整个晶状体浑浊时降低,GSSG-R的活性变化与GSH-PX相似,这两种酶在代谢上是相关的。GSH-S的活性在核浑浊期不改变,但在完全浑浊后降低。

The Study of lens biochemistry.Determination of activities of three enzymes related to glutathione metabolism in cataractous lenses of rats induced by sodium selenite

Activities of GSH peroxidase, GSH reductase and GSH S transferase in catar actous lenses of rats induced by sodium selenite have been determined and compared with normal lenses. It has been found that the activities of GSH peroxidase in nuclear opaque lenses induced by sodium selenite are twice as high as that in the normal lenses, but decreases in the advanced cataractous lenses. The activity of GSH reductase has similar changes in the cataract formation. The data indicate that GSH reductase and GSH peroxidase are metabolically related. The relationship between the activity of these enzymes and cataract formation has been discussed. Our resalts show that GSH S-transferase possibly takes no part in the formation of cataract.