| 蓖麻毒素与其单克隆抗体相互作用动力学研究 |
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| 引用本文： | 高新胜,黄仪秀,朱圣庚,余兴龙,王冬,沈平.蓖麻毒素与其单克隆抗体相互作用动力学研究[J].中国生物化学与分子生物学报,1999,15(5):768-771. |
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| 作者姓名： | 高新胜 黄仪秀 朱圣庚 余兴龙 王冬 沈平 |
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| 作者单位： | 北京大学生命科学学院!北京100871(高新胜，黄仪秀，朱圣庚)，清华大学机械工程学院!北京100084(余兴龙)，北京发玛西亚生物技术中心!北京100101(王冬，沈平) |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 96 70 170 )，高校博士学科点专项科研基金 |
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| 摘 要： | 表面等离子体激元共振(SPR)是一种可微量、实时、动态地监测生物分子相互作用的生物传感技术。蓖麻毒素为核糖体失活蛋白,具有很强的细胞毒性作用。通过SPR技术研究了两种抗蓖麻毒素的单克隆抗体C5、D12与蓖麻毒素相互作用的动力学,计算出两者的亲和常数分别为2.49×108mol-1·L和7.9×108mol-1·L,并对两种抗体的抗原表位进行了分析。
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| 关 键 词： | 蓖麻毒素 表面等离子体激元共振 单克隆抗体 生物分子相互作用 |
| 收稿时间： | 1999-10-20 |
Studies on the Kinetics of the Interaction between Ricin and Its Monoclonal Antibodies |
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| GAO Xinsheng,HUANG Yixiu,ZHU Shenggeng.Studies on the Kinetics of the Interaction between Ricin and Its Monoclonal Antibodies[J].Chinese Journal of Biochemistry and Molecular Biology,1999,15(5):768-771. |
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| Authors: | GAO Xinsheng HUANG Yixiu ZHU Shenggeng |
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| Institution: | (College of Life Sciences,Peking University,Beijing 100871)YU Xinglong(College of Mechanic Engineering,Tsinghua University,Beijing 100084) WANG Dong SHEN Ping(Beijing Pharmacia Biotechnology Centre,Beijing 100101 |
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| Abstract: | Surface Plasmon Resonance(SPR)is a newly developed biosensor technology which can dynamically detect biomolecular interaction in real time Ricin is a kind of ribosome inactivating protein which is exquisitely toxic to eukaryocyte One molecule of ricin in the cytoplasm can kill the cell,so the immunotoxin constructed with ricin can be used in cancer therapy Monoclonal antibodies against ricin can act as the antitoxin to this poison The kinetics of the interaction between ricin and its monoclonal antibodies C5 and D12 were studied via SPR technique and the affinity constants were calculated The K A values for McAb C5 and McAb D12 were 2.49×10 8 mol -1 ·L and 7.9×10 8 mol -1 ·L respectively.The eqitope analysis showed that the two monoclonal antibodies had different epitopes on ricin molecule |
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| Keywords: | Ricin SPR Monoclonal antibody Biomolecular interaction |
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