小鼠单个植入前胚胎SSH方法的可行性

为建立一种能够一次性分离任意两个植入前胚胎之间全部的差别表达的基因的方法 ,在已有的单胚胎操作技术的基础之上 ,对单个植入前胚胎抑制性消减杂交 (singlepreimplantationembryosuppressionsubtractivehybridization ,SPE SSH)方法进行了初步的探索 ,分离到OM2和MⅡ d 2的基因片段 ,经GenBank和文献检索发现 ,这两个基因具有在MⅡ期和 2细胞期特异性表达的特点 .利用cDNA阵列所进行的鉴定也获得了同样的结果 ,而且所使用的材料极少 ,说明SPE SSH是一种强有力的分离和识别早期发育相关基因片段的实验技术 .若与单个卵裂球分离技术相结合 ,还可用于分离和识别人类早期分子诊断的标记性基因

Feasibility of Single Preimplantation Embryonic Suppression Subtractive Hybridization in Mice

In order to systematical analyse the molecular mechanism of the preimplantation development and select the molecular dignostic marker, it is necessary to establish single preimplantation embryo suppression subtractive hybridization (SPE-SSH) to isolate and identify all these differential expressed genes between two random preimplantation samples. After GenBank search and analysis,OM2 and MⅡ-d-2 fragments expressed specifically in MⅡ oocyte and 2-cell embryo were isolated by SPE-SSH. The same results were obtained by cDNA array. It is show that the SPE-SSH is powerful tool for isolation of developmental genes and characterization of molecular dignostic markers.