| PP-1催化亚基（PP-1c）在成体小白鼠不同组织器官中的分化表达与定位 |
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| 引用本文： | 刘文彬,聂磊,惠珊珊,胡小慧,马慧,王玲,周磊,肖亚梅,李万程.PP-1催化亚基（PP-1c）在成体小白鼠不同组织器官中的分化表达与定位[J].中国生物化学与分子生物学报,2011,27(5):437-443. |
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| 作者姓名： | 刘文彬 聂磊 惠珊珊 胡小慧 马慧 王玲 周磊 肖亚梅 李万程 |
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| 作者单位： | 湖南师范大学生命科学学院蛋白质化学与发育生物学教育部重点实验室;Department of Biochemistry and Molecular Biology;Department of Ophthalmology and Visual Sciences;University of Nebraska Medical Center; |
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| 基金项目： | 国家自然科学基金面上项目(No.30971658); 美国NIH基金1R01EY015765和1RO1EY01830; 湖南省芙蓉学者特聘教授基金(No.24030604); 教育厅优秀青年资助项目(No.08B048)资助项目~~ |
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| 摘 要： | 为了确定蛋白磷酸酶-1(protein phosphatase-1)的催化亚基(PP 1c)在小白鼠不同器官组织(肌肉、卵巢、肾、胃、 脾、大脑、心、肝、肺及乳腺)中的表达模式，运用RT-PCR、Western 印迹及荧光免疫组织化学技术等实验手段进行了检测 和分析.结果表明,在mRNA水平， PP-1c在大脑中表达最高，卵巢及肺中表达次之，在肌肉、肾、心、肝中表达较低，在胃 和乳腺中表达最低；在蛋白质水平,肝中表达最高，肾、大脑、肺和乳腺中表达较高，而肌肉、卵巢、心和脾中表达相对较 低，胃中表达最低.免疫荧光组织化学实验结果显示，PP 1c的表达也具有明显的组织特异性和细胞特异性.这些结果为进一 步探讨PP 1在哺乳动物不同组织器官中的功能提供了重要的实验依据.
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| 关 键 词： | 蛋白磷酸酶-1 蛋白磷酸酶-1催化亚基 信号传导 差异表达与定位 |
| 收稿时间： | 2010-12-29 |
Differential Expression and Distribution of the Catalytic Subunits for PP-1 in Different Tissues of Adult Mouse |
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| LIU Wen-Bin,NIE Lei,HUI Shan-Shan,HU Xiao-Hui,MA Hui,WANG Ling,ZHOU Lei,XIAO Ya-Mei,David Wan-cheng LI, Key Laboratory of Protein Chemistry , Development Biology,Ministry of Education,School of Life Sciences,Hunan Normal University,Changsha ,China,.Differential Expression and Distribution of the Catalytic Subunits for PP-1 in Different Tissues of Adult Mouse[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(5):437-443. |
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| Authors: | LIU Wen-Bin NIE Lei HUI Shan-Shan HU Xiao-Hui MA Hui WANG Ling ZHOU Lei XIAO Ya-Mei David Wan-cheng LI Key Laboratory of Protein Chemistry Development Biology Ministry of Education School of Life Sciences Hunan Normal University Changsha China |
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| Institution: | LIU Wen-Bin1),NIE Lei1),HUI Shan-Shan1),HU Xiao-Hui1),MA Hui1),WANG Ling1),ZHOU Lei1),XIAO Ya-Mei1),David Wan-cheng LI2),1),3)(1) Key Laboratory of Protein Chemistry and Development Biology,Ministry of Education,School of Life Sciences,Hunan Normal University,Changsha 410081,China,2) Department of Biochemistry and Molecular Biology,3) Department of Ophthalmology and Visual Sciences,School of Medicine,University of Nebraska Medical Center,Omaha,Nebraska 68198-5870,USA) |
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| Abstract: | To study the differential expression and distribution of the catalytic subunits of protein phosphatase-1(PP-1c) in various tissues of adult mouse,total RNAs and proteins were isolated from the different tissues including muscle,ovary,stomach,kidney,spleen,brain,heart,live,lung and breast.The tissue-specific expression of PP-1c mRNA was analyzed using RT-PCR.The PP-1c protein expression and distribution were examined by Western blot analysis and immunohistochemistry.RT-PCR showed that the mRNAs for PP-1c wer... |
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| Keywords: | protein phosphatase-1 protein phosphatase-1c signal transduction differential expression and distribution |
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