| 猪TCTP基因的表达规律及其对脂肪细胞分化的影响 |
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| 引用本文： | 李新建,杨浩,程佳,宋子仪,杨公社. 猪TCTP基因的表达规律及其对脂肪细胞分化的影响[J]. 中国生物化学与分子生物学报, 2011, 27(5): 444-451 |
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| 作者姓名： | 李新建 杨浩 程佳 宋子仪 杨公社 |
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| 作者单位： | 西北农林科技大学动物脂肪沉积与肌肉发育实验室;河南农业大学畜牧兽医工程学院; |
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| 基金项目： | 转基因生物新品种培育重大专项(No.2009ZX08009-157B)~~ |
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| 摘 要： | 翻译控制肿瘤蛋白（TCTP, translationally controlled tumor protein）是一类广泛存在各种生物、序列高度保守的蛋白，最初认为TCTP是一类生长相关蛋白，近年研究发现TCTP可能具有非常重要的生物学功能.本研究通过高通量测序(Solexa)技术、实时定量PCR (RT qPCR)对瘦肉型和脂肪型猪不同生长阶段脂肪组织、脂肪细胞中TCTP的表达规律进行了研究，采用siRNA技术，沉默TCTP，研究了其对脂肪细胞分化的影响.结果表明：TCTP在瘦肉型猪脂肪组织中的mRNA表达量显著高于脂肪型猪（P＜0.01）|在不同日龄猪脂肪组织中，TCTP的mRNA表达量随着日龄增长而降低|在不同组织中的检测结果发现，TCTP在心、肝、肾、肌肉和脂肪中有较高的表达，肺和脾中表达量较低|TCTP的mRNA表达量在猪前体脂肪细胞增殖过程中逐渐增高，在分化阶段逐渐下降|沉默TCTP促进了脂肪细胞的分化，引起了PPARγ、C/EBPα、SREBP 1c的显著升高（P ＜0.01）.以上研究发现，TCTP在脂肪沉积过程中可能具有抑制作用，为进一步研究肥胖关键基因调控机制提供科学依据.
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| 关 键 词： | 翻译控制肿瘤蛋白 高通量测序 实时定量PCR 猪脂肪组织 siRNA干扰 分化 |
| 收稿时间： | 2010-10-25 |
Expression of Porcine TCTP and Its Effect on Differentiation of Adipocytes |
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| LI Xin-Jian),),YANG Hao),CHENG Jia),SONG Zi-Yi),YANG Gong-She) Laboratory of Animal Fat Deposition , Muscle Development,Northwest A,F University,Yangling ,Shaanxi,China,) School of Animal Husb,ry , Veterinary Science,Henan Agricultural University,Zhengzhou ,Henan,China). Expression of Porcine TCTP and Its Effect on Differentiation of Adipocytes[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(5): 444-451 |
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| Authors: | LI Xin-Jian) ) YANG Hao) CHENG Jia) SONG Zi-Yi) YANG Gong-She) Laboratory of Animal Fat Deposition Muscle Development Northwest A&F University Yangling Shaanxi China ) School of Animal Husb ry & Veterinary Science Henan Agricultural University Zhengzhou Henan China) |
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| Affiliation: | LI Xin-Jian1),2),YANG Hao1),CHENG Jia1),SONG Zi-Yi1),YANG Gong-She1) (1) Laboratory of Animal Fat Deposition and Muscle Development,Northwest A&F University,Yangling 712100,Shaanxi,China,2) School of Animal Husbandry & Veterinary Science,Henan Agricultural University,Zhengzhou 450002,Henan,China) |
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| Abstract: | The translationally controlled tumor protein(TCTP) expressed abundantly in a wide range of organisms from both the animal and plant kingdom,which was initially described as a growth-related protein.However,the studies showed that TCTP has a lot of biological functions.The expression of TCTP in fat tissues of different genotype pigs and in different growth periods were explored by high-throughout sequencing(Solexa) and real-time quantity PCR(RT-qPCR),and its effect on differentiation of adipocytes was explor... |
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| Keywords: | translationally controlled tumor protein high-throughput sequencing RT-qPCR porcine fat tissue siRNA differentiation |
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