EB病毒潜伏膜蛋白1在鼻咽癌中结合磷酸化的TRAFs

 对鼻咽癌中潜伏膜蛋白 1 (LMP1 )是否结合磷酸化的肿瘤坏死因子受体相关因子 (tumornecrosis factor receptor- associated factors,TRAFs)信号分子进行探讨 .首先应用 CSA/SP双染色法在 30例鼻咽癌活检组织中发现 1 6例 (52 % ) LMP1与 TRAF1、TRAF2和 TRAF3共表达于癌细胞胞膜及胞浆同一部位 .这提示 EB病毒 LMP1在鼻咽癌中可能结合 TRAF1、TRAF2或TRAF3发挥作用 .进一步以导入载体 p SG5的鼻咽癌细胞系 HNE2 - p SG5为对照 ,建立了稳定表达 LMP1的鼻咽癌细胞系 HNE2 - LMP1 ,利用这两种细胞系 ,以免疫共沉淀 - Western印迹方法 ,证实了 LMP1可与磷酸化的 TRAF1、TRAF2或 TRAF3直接或间接作用形成免疫共沉淀复合物 .

Phosphotyrosine TRAFs can be Recruited to Epstein-Barr Virus Encoded LMP1 in Nasopharyngeal Carcinoma

Latent membrane protein 1(LMP1)of Epstein\|Barr virus(EBV) plays a critical role in the carcinogenesis of nasopharyngeal carcinoma(NPC).To elucidate the role of LMP1 in NPC carcinogenesis,it was determined whether phosphotyrosine TRAFs could be recruited to LMP1 in NPC.LMP1 positive stable cell line HNE2 LMP1,and a vector control cell line HNF2 pSG5 were established in vitro. Using the HNE2 LMP1 and HNE2 pSG5 cell lines,it was found that the LMP1 bound phosphotyrosine TRAF1,TRAF2 or TRAF3 molecules,suggesting that LMP1 could interact with phosphotyrosine TRAF1,TRAF2 or TRAF3 in HNE2 LMP1 cells.More important,the respective associations of LMP1 with TRAF1,TRAF2 or TRAF3 in 30 NPC tissues were studied by CAS/SP double staining methods.The results showed that LMP1 respectively co\|localizes with TRAF1,TRAF2 or TRAF3 in 16 LMP1 positive samples.All these data suggested EBV LMP1 mediated signaling through the TRAF1,TRAF2 or TRAF3 molecules may be involved in the pathogenesis of the EBV\|positive NPC.