| 五肽胃泌素及其类似物对大鼠胃粘膜细胞DNA合成的作用 |
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| 引用本文： | 王新昌,于志江,陈钧辉,张鹤云,王新光.五肽胃泌素及其类似物对大鼠胃粘膜细胞DNA合成的作用[J].中国生物化学与分子生物学报,1990,6(1):57-60. |
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| 作者姓名： | 王新昌 于志江 陈钧辉 张鹤云 王新光 |
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| 作者单位： | 南京大学生物化学系，南京大学生物化学系，南京大学生物化学系，南京大学生物化学系，南京大学现代分析中心 南京，南京，南京，南京 |
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| 摘 要： | 利用细胞培养的方法,测定五肽胃泌素(Boc-β-Ala-Trp-Met-Asp-phe·NH_2)、葡萄糖-1-脱氧果糖四肽(GIe-1-Deoxyflu-Trp-Met-Asp-phe·NH_2)及Boc-三肽(Boc-Met-Asp-Phe·NH_2)等胃泌素类似物对~3H-胸腺嘧啶核苷(~3H-TdR)参入胃粘膜细胞DNA合成的影响。实验结果表明五肽胃泌素对胃粘膜细胞DNA合成有明显促进作用,而泌酸活性比五肽胃泌素还强的葡萄糖-1-脱氧果糖四肽却没有这种作用。进一步证明胃泌素的营养作用是独立于泌酸活性而存在的,二者有着不同的作用机理。
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| 关 键 词： | 五肽胃泌素 细胞培养 ~3H-胸腺嘧啶核苷 |
| 收稿时间： | 1990-02-20 |
The Effect of Pentagastrin and its Analogs on the DNA Synthesis of Gastric Mucosal Cell From Rat |
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| Wang,Xin-Chang Yu,Zhi-Jiang Chen,Jun-Hui Zhang,He-YunWang,Xin-Guang.The Effect of Pentagastrin and its Analogs on the DNA Synthesis of Gastric Mucosal Cell From Rat[J].Chinese Journal of Biochemistry and Molecular Biology,1990,6(1):57-60. |
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| Authors: | Wang Xin-Chang Yu Zhi-Jiang Chen Jun-Hui Zhang He-YunWang Xin-Guang |
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| Institution: | (Biochemistry Department, Nanjing University, Nanjing) (Modern Analysis Center, Nanjing University |
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| Abstract: | In this paper the cell culture method was used to determine the influences of 3H-Thymidine incorporation into gastric mucosal cell DNA by pentagastrin (Boc-β-Ala-Trp-Met-Asp-Phe·NH2), Glc-1-deoxyf ru-tetrapeptide(Glc -1 - deoxyfru-Trp-Met-Asp-Phe·2NH2) and Boc-tripeptide(Boc-Met-Asp-Phe·NH2). The results in-dica ed that pentagastrin stimulates the DNA synthesis of the rat gastric mucosal cell. However the Glc-1-deoxyfru-Trp-Met-Asp-Phe·NH2 which is stronger than pentagastrin in stimulating gastric acid secretion, does not play a significant role in trophic effect. This suggests that the trophic action of various gastrin analogs is not paralled to its acid-stimulating action. |
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| Keywords: | Pentagastrin Cell culture 3H-Thymidine |
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