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| 胆管癌患者与健康志愿者血清样品蛋白质表达差异分析 | |
| 引用本文： | 练惠辉韩 鹏 邱乒乒康劲翮 戴燕燕李华l亮, 李文岗,陈清西.胆管癌患者与健康志愿者血清样品蛋白质表达差异分析[J].中国生物化学与分子生物学报,2009,25(4):352-357. |
| 作者姓名： | 练惠辉韩 鹏 邱乒乒康劲翮 戴燕燕李华l亮 李文岗 陈清西 |
| 作者单位： | （厦门大学生命科学学院，细胞生物学与肿瘤细胞工程教育部重点实验室，;厦门361005；福建医科大学附属厦门第一医院，厦门361004）;; |
| 摘 要： | 胆管癌（cholangiocarcinoma，CCA）是一种难于早期诊断和治疗的恶性肿瘤.利用蛋白质组学技术以期筛选出人血清中具有医学价值的胆管癌癌变的标志物.利用白蛋白/IgG去除试剂盒去除血清中高丰度的白蛋白和IgG，再经丙酮沉淀得到高质量的血清样品.样品经双向电泳（2-DE）得到了分辨率较好的胆管癌血清的蛋白点图谱.利用Image Master 2D软件对比分析了胆管癌病人和正常健康对照组的2-DE图谱.其中,表达上调蛋白有8个，表达下调蛋白有6个.用质谱获得上述差异蛋白的肽质量指纹图谱，并经数据库检索共鉴定出了11种差 异蛋白质，其中8种蛋白质的表达与胆管癌密切相关.本研究为阐明胆管癌的机理提供了一条新途径. |
| 关 键 词： | 胆管癌 双向电泳 蛋白质组学 血清 |
| 收稿时间： | 2008-9-5 |
Comparative Proteomic Analysis of Serum fromCholangiocarcinoma Patients and Normal Cohorts |
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| LIAN Hui-Hui ,HAN Peng ,QIU Ping-Ping ,KANG Jing-He ,DAI Yan-Yan,LI Hua-Liang ,LI Wen-Gang ,CHEN Qing-Xi .Comparative Proteomic Analysis of Serum fromCholangiocarcinoma Patients and Normal Cohorts[J].Chinese Journal of Biochemistry and Molecular Biology,2009,25(4):352-357. |
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| Authors: | LIAN Hui-Hui HAN Peng QIU Ping-Ping KANG Jing-He DAI Yan-Yan LI Hua-Liang LI Wen-Gang CHEN Qing-Xi |
| Institution: | (1) Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005,China; 2）Xiamen First Hospital Attached to Fujian Medical University, Xiamen 361004,China) |
| Abstract: | The cholangiocarcinoma (CCA) is one of the prevalent cancers in China and often fails early diagnosis and medical intervention. We.performed proteomicanalyses of CCA serological profiles by protein precipitation with acetone after depleting high-abundant seral proteins using an Albumin/IgG Removal Kit. Compared with normal samples, 8 up-regulated and 6 down-regulated proteins were detected in the sera from CCA patients. Within the 11 proteins were identified with mass spectrometry, 8 proteins seemed to be related to CCA by bioinformatic database queries. The results might be for the diagnosis, treatment assessment and prognosis of CCA. |
| Keywords: | cholangiocarcinoma two-dimensional gel electrophoresis proteomics serum |
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