| 美洲拟鲽抗冻蛋白基因(afp)导入番茄的研究 |
| |
| 引用本文： | 黄永芬,汪清胤,付桂荣,赵晓祥,杨志兴.美洲拟鲽抗冻蛋白基因(afp)导入番茄的研究[J].中国生物化学与分子生物学报,1997,13(4):418-422. |
| |
| 作者姓名： | 黄永芬 汪清胤 付桂荣 赵晓祥 杨志兴 |
| |
| 作者单位： | 哈尔滨师范大学生物系，黑龙江省应用微生物研究所 |
| |
| 摘 要： | 将整合在Ｔｉ质粒上的ａｆｐ基因作为供体ＤＮＡ，用花粉管通道和子房注射方法导入番茄品种“中蔬四号”中，对Ｄ１、Ｄ２代进行Ｓｏｕｔｈｅｒｎ杂交获得杂交带；田间抗寒性试验表明，在春季平均气温低于正常年份４·４℃条件下，转基因组植株生长势优于对照；致死温度也比对照降低２℃．说明ａｆｐ基因已整合到转化番茄基因组并获得表达
|
| 关 键 词： | 番茄 美洲拟鲽 抗冻蛋白基因（afp） 外源DNA注射 |
| 收稿时间： | 1997-08-20 |
The Research on Introducing Flounder Antigreeze Protein Gene(afp)into Tomato |
| |
| Huang Yong Fen,Wang Qing Yin,Fu Gui Yong .The Research on Introducing Flounder Antigreeze Protein Gene(afp)into Tomato[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(4):418-422. |
| |
| Authors: | Huang Yong Fen Wang Qing Yin Fu Gui Yong |
| |
| Institution: | (Department of Biology,Harbin Normal University,Harbin 150080) Zhao Xiao Xiang Yang Zhi Xing (Institute of Applied Microbiology Heilongjiang Academy of Sciences,Harbin 15 |
| |
| Abstract: | Antifreeze protein gene(afp)which is integrated into the Ti plasmid pMon322,was introduced into tomato via the pollen tube pathway and ovary injection.The transgenic plants of D 1 and D 2 generation produced afp DNA on Southern blot.The assay of cold resistance in the filed showed that transgenic plants could grow better than control under the average temperature that is 4.4℃ below normal year in earlier spring,meanwhile the fatal temperature dropped 2℃.These results revealed that AFP gene has been integrated into the transgenic plants genome and expressed. |
| |
| Keywords: | ? Tomato Winter flounder Antifreeze protein gene(afp) Pollen tube pathway |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
