| 一株产紫杉醇内生真菌YN6的分离及鉴定 |
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| 引用本文： | 张鹏,刘博,周蓬蓬,王春兰,江晨,余龙江.一株产紫杉醇内生真菌YN6的分离及鉴定[J].中国生物化学与分子生物学报,2011,27(10):961-967. |
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| 作者姓名： | 张鹏 刘博 周蓬蓬 王春兰 江晨 余龙江 |
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| 作者单位： | 华中科技大学生命科学与技术学院资源生物与生物技术研究所;华中科技大学生命科学与技术学院分子生物物理教育部重点实验室;湖北省农业科学研究院经济作物研究所; |
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| 基金项目： | 国家自然科学基金(NSFC No.20776058;20906036); 中国博士后科学基金(No.20100471183)~~ |
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| 摘 要： | 从云南红豆杉（Taxus yunnanensis）树皮内表皮中分离得到75株内生真菌，采用基于紫杉醇合成关键酶10-去乙酰巴卡亭Ⅲ-10-O-乙酰基转移酶（10-deacetylbaccatin Ⅲ-10-O-acetyl transferase,DBAT）和C-13苯丙氨基侧链CoA乙酰基转移酶（C-13-phenylpropanoid side chain-CoA acyltransferase，BAPT）基因为标志分子的快速筛选方法获得一株可产紫杉醇的内生真菌YN6，并通过高效液相色谱法和质谱法对其紫杉醇进行分析.同时，通过对内生真菌YN6的形态特征分析以及18S rDNA序列分析将其初步鉴定为拟盘多毛孢属（Pestalotiopsis sp.）真菌. 拟盘多毛孢YN6的紫杉醇产量约为120~140 μg/L，是目前已报道的紫杉醇产量较高的野生菌株之一. 拟盘多毛孢YN6的发现为微生物发酵生产紫杉醇提供了潜在的优良种质资源.
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| 关 键 词： | ,云南红豆杉,,,内生真菌,,,紫杉醇,,拟盘多毛孢, |
| 收稿时间： | 2011-07-22 |
Isolation and Identification of a Taxol-producing Endophytic Fungus YN6 |
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| ZHANG Peng,,LIU Bo,ZHOU Peng-Peng,WANG Chun-Lan,JIANG Cheng,YU Long-Jiang,.Isolation and Identification of a Taxol-producing Endophytic Fungus YN6[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(10):961-967. |
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| Authors: | ZHANG Peng LIU Bo ZHOU Peng-Peng WANG Chun-Lan JIANG Cheng YU Long-Jiang |
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| Institution: | ZHANG Peng1),2),3),LIU Bo1),ZHOU Peng-Peng1),WANG Chun-Lan1),JIANG Cheng1),YU Long-Jiang1),2)(1)Department of Biotechnology,College of Life Science and Technology,Institute of Resource Biology and Biotechnology,Huazhong University of Science and Technology,Wuhan 430074,China,2)College of Life Science and Technology,Key Laboratory of Molecular Biophysics of Ministry of Education,3) Industrial Crops Institute,Hubei Academy of Agr... |
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| Abstract: | We have isolated endophytic fungi from the inner bark of Taxus yunnanensis. A rapid screening of two key fungi genes for taxol biosynthesis, 10-deacetylbaccatin Ⅲ-10-O-acetyl transferase (DBAT) and C-13 phenylpropanoid side chain-CoA acyltransferase (BAPT), was performed by PCR. One strain taxol producing endophytic fungus (YN6) was obtained and subjected for authentic taxol by high performance liquid chromatography (HPLC) and mass spectrum (MS). Then YN6 was identified to belong to Pestalotiopsis sp. based on morphology and 18S rDNA sequence conservation. YN6 was able to produce 120~140 μg/L taxol and was one of the high-yield taxol-producing wildtype fungi reported. Our finding of Pestalotiopsis sp.YN6 provided a promising strain for producing taxol and could be potentially used for fermentation. |
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| Keywords: | Taxus yunnanensis endophytic fungi taxol Pestalotiopsis |
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