| Lef-1基因在棕色和白色羊驼皮肤差异表达 |
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| 引用本文： | 杨磊,王海东,王祎,于秀菊,董彦君,董常生.Lef-1基因在棕色和白色羊驼皮肤差异表达[J].中国生物化学与分子生物学报,2011,27(11):1061-1066. |
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| 作者姓名： | 杨磊 王海东 王祎 于秀菊 董彦君 董常生 |
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| 作者单位： | 山西农业大学动物科技学院;上海海洋大学水产与生命学院; |
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| 基金项目： | 山西省科技攻关项目(No.2006031060); 山西农业大学创新基金(No.2009001)~~ |
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| 摘 要： | 本实验旨在研究Lef-1在不同毛色羊驼皮肤中的表达和定位,探索其与毛色间的关系.采用实时荧光定量PCR、Western blotting以及免疫组织化学方法,研究白色和棕色羊驼皮肤中的mRNA、蛋白表达水平和定位.实时荧光定量PCR结果显示,Lef-1在棕色羊驼皮肤组织相对基因表达量是3.3727±0.1989，在白色羊驼皮肤组织是1.0003±0.0227; Western blotting结果显示，在羊驼皮肤组织总蛋白中存在分子量约44 KD与兔抗Lef-1多克隆抗体发生免疫阳性反应的蛋白条带，棕色羊驼平均蛋白表达量显著高于白色羊驼；免疫组织化学结果显示，在棕色羊驼皮肤组织中多表达在毛球部、表皮也有分布，在白色羊驼皮肤组织中多表达在表皮，在棕色和白色羊驼皮肤组织中外根鞘都有较强表达.结果显示Lef-1在棕色和白色羊驼皮肤的定位和含量存在差异，提示Lef-1可能影响了羊驼被毛颜色的形成.
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| 关 键 词： | 淋巴增强因子-1(Lef-1) 羊驼 定量实时荧光PCR 蛋白质印迹 免疫组织化学 |
| 收稿时间： | 2011-08-29 |
Differential Expression of Lef-1 Gene in Brown and White Alpaca Skin |
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| YANG Lei,WANG Hai-Dong,WANG Yi,YU Xiu-Ju,DONG Yan-Jun,DONG Chang-ShengCollege of Animal Science , Veterinary Medicine,Shanxi Agricultural University,Shanxi,Taigu ,China,College of Fisheries , Life Science,Shanghai Ocean University,Shanghai ,China.Differential Expression of Lef-1 Gene in Brown and White Alpaca Skin[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(11):1061-1066. |
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| Authors: | YANG Lei WANG Hai-Dong WANG Yi YU Xiu-Ju DONG Yan-Jun DONG Chang-ShengCollege of Animal Science Veterinary Medicine Shanxi Agricultural University Shanxi Taigu China College of Fisheries Life Science Shanghai Ocean University Shanghai China |
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| Institution: | YANG Lei1),WANG Hai-Dong1),WANG Yi2),YU Xiu-Ju1),DONG Yan-Jun1),DONG Chang-Sheng1)(1)College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Shanxi,Taigu 030801,China,2)College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | To study the expression and localization of Lef-1 in alpaca skin of different colors in the process of coat color formation, the mRNA and protein expression levels of Lef-1 were examined by Real-time quantitative PCR, Western blotting and immunohistochemistry. The gene expressive quantity of Lef-1 in the brown alpaca’s skin tissue was 3.3727±0.1989, ones of white alpaca was 1.0003±0.0227. The average protein expression of Lef-1 in brown alpaca’s skin was significantly higher than white alpaca. The molecular weight of Lef-1 protein was about 44 KD. The distribution Lef-1 in alpaca skin were demonstrated, and the distrbution were different between the white and brown alpaca skin. Our results showed that Lef-1 maybe involved in the coat color formation in alpaca. |
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| Keywords: | lymphoid enhancer factor-1(Lef-1) alpaca quantitative real-time PCR(QRT-PCR) Western blotting immunohistochemistry |
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