| 一种简单快速微量的无细胞蛋白合成系统 |
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| 引用本文： | 王润华,沈培奋,李钰云.一种简单快速微量的无细胞蛋白合成系统[J].中国生物化学与分子生物学报,1995,11(4):430-433. |
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| 作者姓名： | 王润华 沈培奋 李钰云 |
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| 作者单位： | 军事医学科学院基础医学研究所 |
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| 摘 要： | 建立了一种简单快速微量的无细胞体系检测蛋白质合成的方法，在总体积５５μｌ的体系中加２０μｌ兔网织红细胞裂解液，在３７℃培养３０ｍｉｎ能使其中的３Ｈ－Ｌｅｕ参入量达到最大，运用该方法可以筛选出植物组织中对真核细胞蛋白质生物合成具有强烈抑制作用的单链核糖体失活蛋白（单链毒素）。
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| 关 键 词： | 无细胞体系 蛋白质生物合成 |
| 收稿时间： | 1995-08-20 |
A Rapid and Simplified Microassay for Measurement of Protein Synthesis in Rabbit Reticulocyte Lysates |
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| Wang,Run-Hua,Shen,Bei-Fen,Li,Yu-Yun.A Rapid and Simplified Microassay for Measurement of Protein Synthesis in Rabbit Reticulocyte Lysates[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(4):430-433. |
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| Authors: | Wang Run-Hua Shen Bei-Fen Li Yu-Yun |
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| Institution: | (Institute of Basic Medical Sciences,Academy of Military Medical Sciences, Beijing 100850 |
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| Abstract: | A rapid and simplified microassay was established for measurement of protein synthesis in rabbit reticulocyte lysates. Maximal3H] leucine incorporation was observed by using 20 μl of reticulocyte lysate per microliter well and by incubating sample for 30 min at 37℃ before harvesting and scintillation counting. By using this microassay,some single chain ribosome-inactivating proteins from plants have been screened for their potential inhibition of protein synthesis in reticulocyte lysates. |
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| Keywords: | Cell-free system Protein synthesis |
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