大豆凝集素的纯化及其凝集不同肿瘤细胞的探讨

大豆凝集素 (SBA)能特异识别N 乙酰氨基半乳糖 (GalNAc)或半乳糖 (Gal) ,引起兔红细胞凝集1] .正常人体细胞表面糖链上的GalNAc或Gal通常被唾液酸分子覆盖 ,不能被SBA识别 .新近研究表明 ,能被SBA特异识别的异常糖链可在多种恶性肿瘤细胞上表达 ,包括 :大鼠乳腺癌细胞系R32 30AC2 ] ,小鼠乳腺肿瘤细胞系TPDMT 4 3 ] ,小鼠T细胞肝转移淋巴瘤L5 178Y F9、SL2 5 4] ,小鼠Lewis肺癌细胞5] ,人类结肠癌细胞系HT2 9、SW12 2 2 6] ,人类胰腺癌细胞系Hup T3、Hup T4 7] ,人类乳腺癌细胞系T 4 7D8] ,附睾乳头状囊腺癌9] …

Purification of Soybean Agglutinin and Its Agglutination Activity Toward Different Cancer Cell Lines

A novel and efficient method for purification of soybean agglutinin (SBA) from soybean was reported. The method was characterized by selective extraction of SBA from soybean homogenate with barbiturate buffer (pH 6 2), removal of impurity by hydroxyapatite, and the final purification of SBA by guaran affinity chromatography. The purified SBA showed a single band of 27 5 kD by SDS PAGE. The lowest concentration of SBA that caused agglutination of the rabbit red blood cells was 0 31 mg/L. Agglutination of different cancer cell lines by the purified SBA was examined. Strong agglutination of the human nasopharyngeal CNE cells, mouse Lewis lung carcinoma cells, and rat mammary adenocarcinoma R3230AC cells was observed. However, SBA could not agglutinate the human hepatocellular carcinoma BEL 7402 cells, suggesting that unlike the above mentioned three cell lines, the BEL 7402 cells may not express N acetylgalactosamine (GalNAc) or galactose (Gal) residues in significant amount at the non reducing terminals of their cell surface glycans.