| 乙型肝炎病毒核心抗原(HBcAg)高表达质粒的构建 |
| |
| 引用本文： | 左平,马贤凯.乙型肝炎病毒核心抗原(HBcAg)高表达质粒的构建[J].中国生物化学与分子生物学报,1985,1(Z1):47-52. |
| |
| 作者姓名： | 左平 马贤凯 |
| |
| 作者单位： | 军事医学科学院基础医学研究所，军事医学科学院基础医学研究所 北京 第二军医大学，北京 |
| |
| 摘 要： | 我们利用了外切酶剪切,人工接头平端连接等DNA重组技术将HBcAg基因片段插入乳糖启动子下游的β-半乳糖苷酶结构基因中,获得了能高效表达HBcAg的重组质粒。ELISA方法检测表明,转化的大肠杆菌每毫升培养物含一万酶联免疫反应单位。
|
| 关 键 词： | DNA重组 乙型肝炎 核心抗原 基因表达 |
| 收稿时间： | 1985-12-20 |
CONSTRUCTION OF PLASMID FOR HIGH LEVEL EXPRESSION OF HBcAG IN E.COLI. |
| |
| Zuo,Ping Ma,Xian-Kai.CONSTRUCTION OF PLASMID FOR HIGH LEVEL EXPRESSION OF HBcAG IN E.COLI.[J].Chinese Journal of Biochemistry and Molecular Biology,1985,1(Z1):47-52. |
| |
| Authors: | Zuo Ping Ma Xian-Kai |
| |
| Institution: | (Intitute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing |
| |
| Abstract: | The recombinant plasmids containing HBcAg gene fragment were constructed by trimming with exonuclease BAL31, blunt end ligation to the synthetic linker, in which HBcAg gene could be highly expressed under the control of the lac promotor. The product was detected by ELISA method, the result shows that it contains ten thousand enzyme immune reactive assay units per mililiter culture of the transformed E. coli. |
| |
| Keywords: | recombinant DNA Hepatitis B Gene expression Core antigen |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
